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PMID: 8871564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins.

Nucleic acids research ·Vol. 24 ·No. 19 ·1996-10-01 ·Pages 3821-8

Funke B, Zuleger B, Benavente R, Schuster T, Goller M, Stévenin J, Horak I

Abstract

The murine poly(C)-binding protein (mCBP) was previously shown to belong to the group of K-homology (KH) proteins by virtue of its homology to hnRNP-K. We have isolated cDNA-splice variants of mCBP which differ by two variable regions of 93 bp and/or 39 +/- 3 bp respectively. Both variable regions are located between the second and third KH-domain of mCBP. The characterization of a partial genomic clone enabled us to propose a model for the generation of the second variable region by the use of a putative alternative splice signal. The mCBP mRNA is expressed ubiquitously and the protein is found predominantly in the nucleus with the exception of the nucleoli. We have identified five proteins which interact with mCBP in the yeast two hybrid system: mouse y-box protein 1 (msy-1), y-box-binding protein, hnRNP-L, filamin and splicing factor 9G8. The interaction between mCBP and splicing factor 9G8 was confirmed in vivo. These results suggest a function of mCBP in RNA metabolism.

MeSH Terms
Animals Isomerism Mice Molecular Sequence Data Nuclear Proteins Nucleocytoplasmic Transport Proteins Poly C/metabolism Protein Binding Pseudogenes RNA Splicing RNA-Binding Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics Serine-Arginine Splicing Factors Subcellular Fractions/metabolism
Chemicals
Nuclear Proteins Nucleocytoplasmic Transport Proteins RNA-Binding Proteins Serine-Arginine Splicing Factors Poly C
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Funke B
Institut für Virologie und Immunbiologie der Universität Würzburg, Germany.
Zuleger B
Benavente R
Schuster T
Goller M
Stévenin J
Horak I
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.19.3821
Published
1996-10-01
Pages
3821-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146158
Subset
IM
Databases
GENBANK
X75947
External Links
PubMed source DOI: 10.1093/nar/24.19.3821 PMC Full Text View journal details
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