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PMID: 8892911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell cycle stage-specific phosphorylation of the Epstein-Barr virus immortalization protein EBNA-LP.

Journal of virology ·Vol. 70 ·No. 11 ·1996-11-00 ·Pages 7885-93

Kitay MK, Rowe DT

Abstract

EBNA-LP is a viral nuclear oncoprotein implicated in the immortalization of B lymphocytes by Epstein-Barr virus. An analysis of EBNA-LP migration on polyacrylamide gels was performed with protein derived from the X50-7 lymphoblastoid cell line blocked by hydroxyurea or aphidicolin at the G1/S phase of the cell cycle or by nocodazole at the G2/M phase. More slowly migrating species of EBNA-LP were detected in G2/M phase-arrested cell extracts. Release from nocodazole G2/M block or treatment with phosphatase caused the more slowly migrating species of EBNA-LP to disappear. Analyses of 32PO(4)(3-)-labeled EBNA-LP protein immunoprecipitated from the drug-synchronized cells showed that phosphorylated EBNA-LP was present throughout the cell cycle but that phosphorylation increased in G2 and was maximal at G2/M. Phosphoamino acid analysis revealed that all phosphorylation was on serine residues only. The ability of EBNA-LP to be phosphorylated by p34(cdc2) kinase and casein kinase II exclusively on serines implicates these enzymes as being potentially involved in EBNA-LP phosphorylation.

MeSH Terms
Binding Sites CDC2 Protein Kinase/metabolism Casein Kinase II Cell Cycle Cell Line Electrophoresis, Polyacrylamide Gel Epstein-Barr Virus Nuclear Antigens/metabolism Herpesvirus 4, Human/metabolism Humans Peptide Mapping Phosphorylation Protein Serine-Threonine Kinases/metabolism Tumor Cells, Cultured
Chemicals
Epstein-Barr Virus Nuclear Antigens Casein Kinase II Protein Serine-Threonine Kinases CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kitay M K
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261, USA.
Rowe D T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.11.7885-7893.1996
Published
1996-11-00
Pages
7885-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190860
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.11.7885-7893.1996 PMC Full Text View journal details
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