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PMID: 8895581 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.

The EMBO journal ·Vol. 15 ·No. 19 ·1996-10-01 ·Pages 5370-82

Wang W, Côté J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL, Crabtree GR

Abstract

We have purified distinct complexes of nine to 12 proteins [referred to as BRG1-associated factors (BAFs)] from several mammalian cell lines using an antibody to the SWI2-SNF2 homolog BRG1. Microsequencing revealed that the 47 kDa BAF is identical to INI1. Previously INI1 has been shown to interact with and activate human immunodeficiency virus integrase and to be homologous to the yeast SNF5 gene. A group of BAF47-associated proteins were affinity purified with antibodies against INI1/BAF47 and were found to be identical to those co-purified with BRG1, strongly indicating that this group of proteins associates tightly and is likely to be the mammalian equivalent of the yeast SWI-SNF complex. Complexes containing BRG1 can disrupt nucleosomes and facilitate the binding of GAL4-VP16 to a nucleosomal template similar to the yeast SWI-SNF complex. Purification of the complex from several cell lines demonstrates that it is heterogeneous with respect to subunit composition. The two SWI-SNF2 homologs, BRG1 and hbrm, were found in separate complexes. Certain cell lines completely lack BRG1 and hbrm, indicating that they are not essential for cell viability and that the mammalian SWI-SNF complex may be tailored to the needs of a differentiated cell type.

MeSH Terms
Amino Acid Sequence Animals Cell Line Chromosomal Proteins, Non-Histone DNA Helicases DNA-Binding Proteins/chemistry,isolation & purification,metabolism Humans Mammals Mice Molecular Sequence Data Molecular Weight Nuclear Proteins/chemistry,metabolism Nucleosomes/metabolism Rats Recombinant Fusion Proteins/metabolism SMARCB1 Protein Sequence Homology, Amino Acid Species Specificity Transcription Factors/chemistry,isolation & purification,metabolism Transcriptional Activation
Chemicals
Chromosomal Proteins, Non-Histone DNA-Binding Proteins Nuclear Proteins Nucleosomes Recombinant Fusion Proteins SMARCA2 protein, human SMARCB1 Protein SMARCB1 protein, human Smarca2 protein, mouse Smarcb1 protein, mouse Transcription Factors SMARCA4 protein, human Smarca4 protein, mouse DNA Helicases
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Wang W
Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, CA 94305-5428, USA.
Côté J
Xue Y
Zhou S
Khavari P A
Biggar S R
Muchardt C
Kalpana G V
Goff S P
Yaniv M
Workman J L
Crabtree G R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-10-01
Pages
5370-82
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452280
Subset
IM
Grants
NIGMS NIH HHS · GM47867 · United States
External Links
PubMed source PMC Full Text View journal details
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