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PMID: 8943030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intron of the mouse Hoxa-7 gene contains conserved homeodomain binding sites that can function as an enhancer element in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 24 ·1996-11-26 ·Pages 13884-9

Haerry TE, Gehring WJ

Abstract

The 5' flanking sequences and the intron of the mouse Hoxa-7 gene were searched for regulatory elements that can function in Drosophila. Only the intron is able to activate a lacZ fusion gene in various tissues of Drosophila embryos. This enhancer function requires a cluster of three homeodomain binding sites (HB1-element) that are also found in the introns of other Hox genes as well as in a putative autoregulatory element of Ultrabithorax (Ubx), the Drosophila homolog of Hoxa-7. If a single binding site in the autoregulatory element of fushi tarazu (ftz) is replaced by the HB1-element of Hoxa-7, the expression pattern is altered and newly controlled by the homeotic gene caudal (cad). These data suggest that HB1 is a potential target for different homeodomain proteins of both vertebrates and invertebrates.

MeSH Terms
Animals Base Sequence Binding Sites DNA-Binding Proteins/biosynthesis,genetics Drosophila/embryology,genetics,physiology Drosophila Proteins Drosophila melanogaster/genetics,physiology Embryo, Nonmammalian/physiology Enhancer Elements, Genetic Fushi Tarazu Transcription Factors Gene Expression Regulation, Developmental Genes, Homeobox Homeodomain Proteins/biosynthesis,genetics Humans Introns Mice/genetics Molecular Sequence Data Sequence Homology, Nucleic Acid Transcription Factors
Chemicals
DNA-Binding Proteins Drosophila Proteins Fushi Tarazu Transcription Factors Homeodomain Proteins Transcription Factors Ubx protein, Drosophila ftz protein, Drosophila
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haerry T E
Biozentrum, University of Basel, Switzerland.
Gehring W J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.24.13884
Published
1996-11-26
Pages
13884-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19457
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.24.13884 PMC Full Text View journal details
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