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PMID: 8976182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Experimental autoimmune encephalomyelitis induction in genetically B cell-deficient mice.

The Journal of experimental medicine ·Vol. 184 ·No. 6 ·1996-12-01 ·Pages 2271-8

Wolf SD, Dittel BN, Hardardottir F, Janeway CA

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an animal model for autoimmune central nervous system disease mediated by CD4 T cells. To examine the role of B cells in the induction of EAE, we used B10.PL (I-Au) mice rendered deficient in B cells by deletion of their mu chain transmembrane region (B10.PLmicroMT). By immunizing B10.PL and B10.PLmicroMT mice with the NH-terminal myelin basic protein encephalitogenic peptide Ac1-11, we observed no difference in the onset or severity of disease in the absence of mature B cells. There was, however, a greater variation in disease onset, severity, and especially of recovery in the B cell-deficient mice compared to controls. B10.PLmicroMT mice rarely returned to normal in the absence of B cells. Taken together, our data suggest that B cells do not play a role in the activation of encephalitogenic T cells, but may contribute to the immune modulation of acute EAE. The mechanisms to explain these effects are discussed.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/immunology Base Sequence Cytokines/biosynthesis DNA Primers Encephalomyelitis, Autoimmune, Experimental/immunology Flow Cytometry Immunoglobulin mu-Chains/genetics Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Mutant Strains Molecular Sequence Data Peptide Fragments/chemical synthesis,immunology Polymerase Chain Reaction RNA, Messenger/biosynthesis Sequence Deletion T-Lymphocytes/immunology Transcription, Genetic
Chemicals
Cytokines DNA Primers Immunoglobulin mu-Chains Peptide Fragments RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolf S D
Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Dittel B N
Hardardottir F
Janeway C A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.6.2271
Published
1996-12-01
Pages
2271-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2196394
Subset
IM
Grants
NIAID NIH HHS · AI-36529 · United States
External Links
PubMed source DOI: 10.1084/jem.184.6.2271 PMC Full Text View journal details
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