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PMID: 9155040 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromatin structure modulates DNA repair by photolyase in vivo.

The EMBO journal ·Vol. 16 ·No. 8 ·1997-04-15 ·Pages 2150-60

Suter B, Livingstone-Zatchej M, Thoma F

Abstract

Yeast and many other organisms use nucleotide excision repair (NER) and photolyase in the presence of light (photoreactivation) to repair cyclobutane pyrimidine dimers (CPDs), a major class of DNA lesions generated by UV light. To study the role of photoreactivation at the chromatin level in vivo, we used yeast strains which contained minichromosomes (YRpTRURAP, YRpCS1) with well-characterized chromatin structures. The strains were either proficient (RAD1) or deficient (rad1 delta) in NER. In contrast to NER, photolyase rapidly repairs CPDs in non-nucleosomal regions, including promoters of active genes (URA3, HIS3, DED1) and in linker DNA between nucleosomes. CPDs in nucleosomes are much more resistant to photoreactivation. These results demonstrate a direct role of chromatin in modulation of a DNA repair process and an important role of photolyase in repair of damaged promoters with presumptive effects on gene regulation. In addition, photoreactivation provides an in vivo test for chromatin structure and stability. In active genes (URA3, HIS3), photolyase repairs the non-transcribed strand faster than the transcribed strand and can match fast removal of lesions from the transcribed strand by NER (transcription-coupled repair). Thus, the combination of both repair pathways ensures efficient repair of active genes.

MeSH Terms
Chromatin/chemistry Chromosomes, Fungal DNA Damage DNA Repair/physiology,radiation effects DNA Repair Enzymes DNA-Binding Proteins Deoxyribodipyrimidine Photo-Lyase/metabolism Endonucleases/genetics Fungal Proteins/genetics Light Models, Genetic Mutation Nucleosomes Promoter Regions, Genetic Pyrimidine Dimers Saccharomyces cerevisiae/genetics,radiation effects Saccharomyces cerevisiae Proteins Ultraviolet Rays
Chemicals
Chromatin DNA-Binding Proteins Fungal Proteins Nucleosomes Pyrimidine Dimers Saccharomyces cerevisiae Proteins Endonucleases RAD1 protein, S cerevisiae Deoxyribodipyrimidine Photo-Lyase DNA Repair Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Suter B
Institut für Zellbiologie, ETH-Hönggerberg, Zürich, Switzerland.
Livingstone-Zatchej M
Thoma F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.8.2150
Published
1997-04-15
Pages
2150-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169817
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.8.2150 PMC Full Text View journal details
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