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PMID: 9184851 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Autophagic proteolysis: control and specificity.

The Histochemical journal ·Vol. 29 ·No. 5 ·1997-05-00 ·Pages 365-85

Blommaart EF, Luiken JJ, Meijer AJ

Abstract

The rate of proteolysis is an important determinant of the intracellular protein content. Part of the degradation of intracellular proteins occurs in the lysosomes and is mediated by macroautophagy. In liver, macroautophagy is very active and almost completely accounts for starvation-induced proteolysis. Factors inhibiting this process include amino acids, cell swelling and insulin. In the mechanisms controlling macroautophagy, protein phosphorylation plays an important role. Activation of a signal transduction pathway, ultimately leading to phosphorylation of ribosomal protein S6, accompanies inhibition of macroautophagy. Components of this pathway may include a heterotrimeric Gi3-protein, phosphatidylinositol 3-kinase and p70S6 kinase. Recent evidence indicates that lysosomal protein degradation can be selective and occurs via ubiquitin-dependent and -independent pathways.

MeSH Terms
Amino Acids/metabolism Animals Autophagy/drug effects,physiology Cell Size Cytosol/metabolism Humans Insulin/metabolism Insulin Secretion Liver/cytology,metabolism,ultrastructure Lysosomes/drug effects,metabolism,ultrastructure Microscopy, Electron Neoplasms/metabolism,pathology Phosphoprotein Phosphatases/metabolism Phosphorylation Protein Kinases/metabolism Proteins/metabolism Ribosomal Proteins/metabolism Sensitivity and Specificity Signal Transduction/drug effects,physiology Ubiquitins/pharmacology
Chemicals
Amino Acids Insulin Proteins Ribosomal Proteins Ubiquitins Protein Kinases Phosphoprotein Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blommaart E F
Department of Biochemistry, University of Amsterdam, The Netherlands.
Luiken J J
Meijer A J
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Article Info
Journal
The Histochemical journal
Abbr.
Histochem J
ISSN
0018-2214
DOI
10.1023/a:1026486801018
Published
1997-05-00
Pages
365-85
Language
English
Region
Netherlands
NLM ID
0163161
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1026486801018
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