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PMID: 9199338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Small heat shock protein suppression of Vpr-induced cytoskeletal defects in budding yeast.

Molecular and cellular biology ·Vol. 17 ·No. 7 ·1997-07-00 ·Pages 4033-42

Gu J, Emerman M, Sandmeyer S

Abstract

Expression of the auxiliary human immunodeficiency virus type 1 (HIV-1) protein Vpr causes arrest of primate host cells in G2. Expression of this protein in budding yeast has been previously reported to cause growth arrest and a large-cell phenotype. Investigation of the effect of Vpr expression in budding yeast, reported here, showed that it causes disruption of the actin cytoskeleton. Expression of HSP42, the gene for a small heat shock protein (sHSP), from a high-copy-number plasmid reversed this effect. The sHSPs are induced by exposure of cells to thermal, osmotic, and oxidative stresses and to mitogens. In animal cells, overexpression of sHSPs causes increased resistance to stress and stabilization of actin stress fibers. Yeast cells subjected to mild stress, such as shifting from 23 to 39 degrees C, arrest growth and then resume cell division. Growth arrest is accompanied by transient disorganization of the cytoskeleton. Yeast in which the HSP42 gene was disrupted and which was subjected to moderate thermal stress reorganized the actin cytoskeleton more slowly than did wild-type control cells. These results demonstrate that in yeast, as in metazoan cells, sHSPs promote maintenance of the actin cytoskeleton.

MeSH Terms
Actin Cytoskeleton/ultrastructure Actins/metabolism Gene Expression Regulation, Fungal Gene Expression Regulation, Viral Gene Products, vpr/physiology Heat Stress Disorders/pathology Heat-Shock Proteins/physiology Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Chemicals
Actins Gene Products, vpr HSP42 protein, S cerevisiae Heat-Shock Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gu J
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92697-4025, USA.
Emerman M
Sandmeyer S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.7.4033
Published
1997-07-00
Pages
4033-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232256
Subset
IM
Grants
NIAID NIH HHS · R01 AI30927 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.7.4033 PMC Full Text View journal details
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