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PMID: 9199347 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development.

Molecular and cellular biology ·Vol. 17 ·No. 7 ·1997-07-00 ·Pages 4114-23

Ashery-Padan R, Ulitzur N, Arbel A, Goldberg M, Weiss AM, Maus N, Fisher PA, Gruenbaum Y

Abstract

Otefin is a peripheral protein of the inner nuclear membrane in Drosophila melanogaster. Here we show that during nuclear assembly in vitro, it is required for the attachment of membrane vesicles to chromatin. With the exception of sperm cells, otefin colocalizes with lamin Dm0 derivatives in situ and presumably in vivo and is present in all somatic cells examined during the different stages of Drosophila development. In the egg chamber, otefin accumulates in the cytoplasm, in the nuclear periphery, and within the nucleoplasm of the oocyte, in a pattern similar to that of lamin Dm0 derivatives. There is a relatively large nonnuclear pool of otefin present from stages 6 to 7 of egg chamber maturation through 6 to 8 h of embryonic development at 25 degrees C. In this pool, otefin is peripherally associated with a fraction containing the membrane vesicles. This association is biochemically different from the association of otefin with the nuclear envelope. Otefin is a phosphoprotein in vivo and is a substrate for in vitro phosphorylation by cdc2 kinase and cyclic AMP-dependent protein kinase. A major site for cdc2 kinase phosphorylation in vitro was mapped to serine 36 of otefin. Together, our data suggest an essential role for otefin in the assembly of the Drosophila nuclear envelope.

MeSH Terms
Animals CDC2 Protein Kinase/metabolism Cell Compartmentation Chromatin/metabolism Cyclic AMP-Dependent Protein Kinases/metabolism Drosophila Proteins Drosophila melanogaster/embryology Fluorescent Antibody Technique, Indirect Insect Proteins/metabolism Lamins Membrane Proteins/metabolism Nuclear Envelope/ultrastructure Nuclear Proteins/metabolism Phosphoproteins/metabolism Phosphorylation Phosphoserine/metabolism Protein Processing, Post-Translational
Chemicals
Chromatin Drosophila Proteins Insect Proteins Lam protein, Drosophila Lamins Membrane Proteins Nuclear Proteins Ote protein, Drosophila Phosphoproteins Phosphoserine Cyclic AMP-Dependent Protein Kinases CDC2 Protein Kinase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ashery-Padan R
Department of Genetics, The Life Sciences Institute, The Hebrew University of Jerusalem, Israel.
Ulitzur N
Arbel A
Goldberg M
Weiss A M
Maus N
Fisher P A
Gruenbaum Y
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.7.4114
Published
1997-07-00
Pages
4114-23
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232265
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.7.4114 PMC Full Text View journal details
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