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PMID: 9218801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High level transcription of a member of a repeated gene family confers dehydration tolerance to callus tissue of Craterostigma plantagineum.

The EMBO journal ·Vol. 16 ·No. 12 ·1997-06-16 ·Pages 3599-608

Furini A, Koncz C, Salamini F, Bartels D

Abstract

An experimental system has been developed which allows the identification of intermediates in the abscisic acid (ABA) signal transduction pathway leading to desiccation tolerance in plants. Desiccation tolerance in callus of the resurrection plant Craterostigma plantagineum is mediated via the plant hormone ABA, which induces the expression of gene products related to desiccation tolerance. Based on T-DNA activation tagging, a gene (CDT-1) was isolated which encodes a signalling molecule in the ABA transduction pathway. Constitutive overexpression of CDT-1 leads to desiccation tolerance in the absence of ABA and to the constitutive expression of characteristic transcripts. CDT-1 represents a novel gene with unusual features in its primary sequence. The CDT-1 gene resembles in several features SINE retrotransposons. Mechanisms by which CDT-1 activates the pathway could be via a regulatory RNA or via a short polypeptide.

MeSH Terms
Abscisic Acid/metabolism Adaptation, Physiological/genetics Amino Acid Sequence Base Sequence Chromosome Mapping DNA, Plant Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Multigene Family Mutation Plant Proteins/genetics Plants RNA, Plant Signal Transduction Transcription, Genetic
Chemicals
CDT-1 protein, Craterostigma plantagineum DNA, Plant Plant Proteins RNA, Plant Abscisic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Furini A
Max-Planck-Institüt für Zuchtungsforschung, Köln, Germany.
Koncz C
Salamini F
Bartels D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.12.3599
Published
1997-06-16
Pages
3599-608
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169984
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.12.3599 PMC Full Text View journal details
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