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PMID: 9234743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex.

Molecular and cellular biology ·Vol. 17 ·No. 8 ·1997-08-00 ·Pages 4870-6

Kiledjian M, DeMaria CT, Brewer G, Novick K

Abstract

mRNA turnover is an important regulatory component of gene expression and is significantly influenced by ribonucleoprotein (RNP) complexes which form on the mRNA. Studies of human alpha-globin mRNA stability have identified a specific RNP complex (alpha-complex) which forms on the 3' untranslated region (3'UTR) of the mRNA and appears to regulate the erythrocyte-specific accumulation of alpha-globin mRNA. One of the protein activities in this multiprotein complex is a poly(C)-binding activity which consists of two proteins, alphaCP1 and alphaCP2. Neither of these proteins, individually or as a pair, can bind the alpha-globin 3'UTR unless they are complexed with the remaining non-poly(C) binding proteins of the alpha-complex. With the yeast two-hybrid screen, a second alpha-complex protein was identified. This protein is a member of the previously identified A+U-rich (ARE) binding/degradation factor (AUF1) family of proteins, which are also known as the heterogeneous nuclear RNP (hnRNP) D proteins. We refer to these proteins as AUF1/hnRNP-D. Thus, a protein implicated in ARE-mediated mRNA decay is also an integral component of the mRNA stabilizing alpha-complex. The interaction of AUF1/hnRNP-D is more efficient with alphaCP1 relative to alphaCP2 both in vitro and in vivo, suggesting that the alpha-complex might be dynamic rather than a fixed complex. AUF1/hnRNP-D could, therefore, be a general mRNA turnover factor involved in both stabilization and decay of mRNA.

MeSH Terms
DNA-Binding Proteins Globins/genetics Heterogeneous Nuclear Ribonucleoprotein D0 Heterogeneous-Nuclear Ribonucleoprotein D Heterogeneous-Nuclear Ribonucleoproteins Humans Leukemia, Erythroblastic, Acute Macromolecular Substances Multiprotein Complexes Poly C/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism Recombinant Fusion Proteins/metabolism Ribonucleoproteins/metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins GAL4 protein, S cerevisiae HNRNPD protein, human Heterogeneous Nuclear Ribonucleoprotein D0 Heterogeneous-Nuclear Ribonucleoprotein D Heterogeneous-Nuclear Ribonucleoproteins Macromolecular Substances Multiprotein Complexes PCBP1 protein, human RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins Ribonucleoproteins Saccharomyces cerevisiae Proteins Transcription Factors Poly C Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kiledjian M
Department of Cell, Developmental and Neurobiology, Rutgers University, Piscataway, New Jersey 08855, USA. [email protected]
DeMaria C T
Brewer G
Novick K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.8.4870
Email
[email protected]
Published
1997-08-00
Pages
4870-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232339
Subset
IM
Grants
NCI NIH HHS · CA52443 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/MCB.17.8.4870 PMC Full Text View journal details
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