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PMID: 9246412 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of alien chromatin conferring resistance to the beet cyst nematode (Heterodera schachtii Schm.) in cultivated beet (Beta vulgaris L.) using in situ hybridization.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 5 ·No. 3 ·1997-05-00 ·Pages 186-93

Schmidt T, Jung C, Heslop-Harrison JS, Kleine M

Abstract

Chromatin originating from wild beets of the genus Beta, section Procumbentes, has been investigated in nematode-resistant hybrid-derived lines of sugar beet (Beta vulgaris L.) by in situ hybridization using satellite, telomeric and ribosomal DNA repeats, a yeast artificial chromosome (YAC) and total genomic DNA as probes. The allen chromosome was detected in three monosomic addition lines (2n = 18 + 1) by genomic in situ hybridization. Fluorescence in situ hybridization with a genome-specific satellite repeat and YAC DNA enabled the visualization of Procumbentes chromosomes, and in double-target hybridization it was shown that they do not carry 18S-5.8S-25S rRNA and 5S rRNA genes. The wild beet-specific satellite repeat and the telomere sequence from Arabidopsis thaliana were used to perform a structural analysis of the wild beet chromosome fragments of two resistant fragment addition lines. It was shown that one physical end of the chromosome fragments consists of telomeric repeats. Comparison of fragment sizes indicated that the small chromosome fragments harbouring the resistance gene most likely resulted from the loss of one wild beet chromosome arm and an internal deletion of the remaining arm.

MeSH Terms

Chimera DNA Probes DNA, Ribosomal DNA, Satellite In Situ Hybridization, Fluorescence Nematode Infections/genetics Plant Diseases/genetics RNA, Ribosomal, 18S/genetics RNA, Ribosomal, 28S/genetics RNA, Ribosomal, 5.8S/genetics Repetitive Sequences, Nucleic Acid Telomere Vegetables/genetics,parasitology

Chemicals

DNA Probes DNA, Ribosomal DNA, Satellite RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Ribosomal, 5.8S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schmidt T

Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Germany.

Jung C

Heslop-Harrison J S

Kleine M

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Article Info

Journal

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Abbr.

Chromosome Res

ISSN

0967-3849

DOI

10.1023/a:1018447031020

Published

1997-05-00

Pages

186-93

Language

English

Region

Netherlands

NLM ID

9313452

Subset

External Links

PubMed source DOI: 10.1023/a:1018447031020 View journal details

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Detection of alien chromatin conferring resistance to the beet cyst nematode (Heterodera schachtii Schm.) in cultivated beet (Beta vulgaris L.) using in situ hybridization.

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