Home LiteratureArticle Details
PMID: 9286999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.

Journal of bacteriology ·Vol. 179 ·No. 17 ·1997-09-00 ·Pages 5442-7

Cornillot E, Nair RV, Papoutsakis ET, Soucaille P

Abstract

Degeneration is the process whereby Clostridium acetobutylicum ATCC 824 loses the capacity to produce acetone and butanol after repeated vegetative transfers or in continuous culture. Two degenerate mutants (M5 and DG1) of C. acetobutylicum ATCC 824 do not contain the four genes (ctfA, ctfB, adc, and aad) for acetone and butanol formation. Strain ATCC 824 contains a 210-kb plasmid (pSOL1) which is absent in M5 and DG1. pSOL1 carries the four acetone and butanol formation genes. A restriction map of pSOL1 was constructed by using ApaI, SmaI, SstII, and NarI digestions. M5 and DG1 could be complemented for acetone and butanol formation by expressing the corresponding genes (ctfA, ctfB, and adc for acetone; aad for butanol) on the plasmid. Degeneration of this strain thus appears to be the result of pSOL1 loss.

MeSH Terms
Acetone/metabolism Alcohol Oxidoreductases/genetics Aldehyde Oxidoreductases/genetics Bacterial Proteins/genetics Butanols/metabolism Carboxy-Lyases/genetics Clostridium/enzymology,genetics,metabolism Coenzyme A-Transferases/genetics Conjugation, Genetic Genes, Bacterial/genetics Multienzyme Complexes/genetics Operon/genetics Plasmids/genetics,metabolism Restriction Mapping
Chemicals
Bacterial Proteins Butanols Multienzyme Complexes Acetone AAD protein, Clostridium acetobutylicum Alcohol Oxidoreductases Aldehyde Oxidoreductases Coenzyme A-Transferases ctfA protein, Clostridium acetobutylicum ctfB protein, Clostridium acetobutylicum Carboxy-Lyases acetoacetate decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cornillot E
INSA, Centre de Bioingénierie Gilbert Durand, Complexe Scientifique deRangueil, Toulouse, France.
Nair R V
Papoutsakis E T
Soucaille P
References (24)
24 references, click to expand
  1. Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum.
    J Bacteriol. 1990 Dec;172(12):6907-18 PMID: 2254264
  2. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.
    Plasmid. 1978 Sep;1(4):584-8 PMID: 107540
  3. Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis.
    Nucleic Acids Res. 1988 Feb 11;16(3):925-39 PMID: 3344223
  4. Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis.
    J Bacteriol. 1993 Nov;175(21):6959-69 PMID: 8226639
  5. Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824.
    Microbiology. 1996 Aug;142 ( Pt 8):2079-86 PMID: 8760920
  6. Taxonomy and phylogeny of industrial solvent-producing clostridia.
    Int J Syst Bacteriol. 1995 Oct;45(4):693-705 PMID: 7547288
  7. Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis.
    J Bacteriol. 1986 Jul;167(1):138-47 PMID: 3087948
  8. Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.
    J Bacteriol. 1994 Sep;176(18):5843-6 PMID: 8083176
  9. Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways.
    Mol Microbiol. 1993 Sep;9(5):923-9 PMID: 7934920
  10. Pulsed field separation of large supercoiled and open-circular DNAs and its application to bacterial artificial chromosome cloning.
    Electrophoresis. 1995 Jan;16(1):1-7 PMID: 7737080
  11. Transductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2.
    Appl Environ Microbiol. 1976 Jul;32(1):45-52 PMID: 823867
  12. Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.
    Gene. 1993 Jan 15;123(1):93-7 PMID: 8423010
  13. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  14. Solvent Production and Morphological Changes in Clostridium acetobutylicum.
    Appl Environ Microbiol. 1982 Jun;43(6):1434-9 PMID: 16346038
  15. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  16. DNA sequences specifying the transcription of the streptococcal kanamycin resistance gene in Escherichia coli and Bacillus subtilis.
    Mol Gen Genet. 1985;198(2):348-52 PMID: 3920478
  17. Comparative metabolism of vegetative and sporulating cultures of Clostridium thermosaccharolyticum.
    J Bacteriol. 1970 May;102(2):369-76 PMID: 4315892
  18. Isolation and Characterization of Mutants of Clostridium acetobutylicum ATCC 824 Deficient in Acetoacetyl-Coenzyme A:Acetate/Butyrate:Coenzyme A-Transferase (EC 2.8.3.9) and in Other Solvent Pathway Enzymes.
    Appl Environ Microbiol. 1989 Apr;55(4):970-6 PMID: 16347898
  19. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.
    J Bacteriol. 1994 Feb;176(3):871-85 PMID: 8300540
  20. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  21. Acetone-butanol fermentation revisited.
    Microbiol Rev. 1986 Dec;50(4):484-524 PMID: 3540574
  22. Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
    J Bacteriol. 1985 Apr;162(1):328-34 PMID: 3884593
  23. Cosmid mapping of the human chorionic gonadotropin beta subunit genes by field-inversion gel electrophoresis.
    Nucleic Acids Res. 1987 Jun 11;15(11):4437-48 PMID: 3035494
  24. Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli.
    Appl Environ Microbiol. 1990 Jun;56(6):1576-83 PMID: 2383002
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.17.5442-5447.1997
Published
1997-09-00
Pages
5442-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179415
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.179.17.5442-5447.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2