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PMID: 9307973 Published · ppublish English Journal Article

An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal.

Molecular biology of the cell ·Vol. 8 ·No. 9 ·1997-09-00 ·Pages 1777-87

Hui N, Nakamura N, Sönnichsen B, Shima DT, Nilsson T, Warren G

Abstract

The early Golgi t-SNARE (target-membrane-associated soluble-N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 5 is thought to specify the docking site for both COPI and COPII coated vesicles originating from the endoplasmic reticulum (ER) and COPI vesicles on the retrograde pathway. We now show that there are two forms of syntaxin 5 that appear to be generated from the same mRNA by alternative initiation of translation. The short form (35 kDa) corresponds to the published sequence. The long form (42 kDa) has an N-terminal cytoplasmic extension containing a predicted type II ER retrieval signal. When grafted onto a reporter molecule, this signal localized the construct to the ER. Biochemical fractionation and immunofluorescence microscopy showed that there was less of the long form in the Golgi apparatus and more in peripheral punctate structures, some of which colocalized with markers of the intermediate compartment. The predicted absence of the long form in budding yeast points to a function unique to higher organisms.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Endoplasmic Reticulum/physiology Golgi Apparatus/chemistry,metabolism,physiology Humans Isomerism Liver/chemistry Membrane Proteins/chemistry,genetics,physiology Molecular Sequence Data Molecular Weight Qa-SNARE Proteins Rats Rats, Sprague-Dawley SNARE Proteins Signal Transduction Vesicular Transport Proteins
Chemicals
Membrane Proteins Qa-SNARE Proteins SNARE Proteins Vesicular Transport Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hui N
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Nakamura N
Sönnichsen B
Shima D T
Nilsson T
Warren G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.8.9.1777
Published
1997-09-00
Pages
1777-87
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC305736
Subset
IM
Databases
GENBANK
U87971
External Links
PubMed source DOI: 10.1091/mbc.8.9.1777 PMC Full Text View journal details
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