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PMID: 9334400 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 21 ·1997-11-01 ·Pages 8246-58

Rhodes KJ, Strassle BW, Monaghan MM, Bekele-Arcuri Z, Matos MF, Trimmer JS

Abstract

The differential expression and association of cytoplasmic beta-subunits with pore-forming alpha-subunits may contribute significantly to the complexity and heterogeneity of voltage-gated K+ channels in excitable cells. Here we examined the association and colocalization of two mammalian beta-subunits, Kvbeta1 and Kvbeta2, with the K+ channel alpha-subunits Kv1.1, Kv1.2, Kv1.4, Kv1.6, and Kv2.1 in adult rat brain. Reciprocal coimmunoprecipitation experiments using subunit-specific antibodies indicated that Kvbeta1 and Kvbeta2 associate with all the Kv1 alpha-subunits examined, and with each other, but not with Kv2.1. A much larger portion of the total brain pool of Kv1-containing channel complexes was found associated with Kvbeta2 than with Kvbeta1. Single- and multiple-label immunohistochemical staining indicated that Kvbeta1 codistributes extensively with Kv1.1 and Kv1.4 in cortical interneurons, in the hippocampal perforant path and mossy fiber pathways, and in the globus pallidus and substantia nigra. Kvbeta2 codistributes extensively with Kv1.1 and Kv1.2 in all brain regions examined and was strikingly colocalized with these alpha-subunits in the juxtaparanodal region of nodes of Ranvier as well as in the axons and terminals of cerebellar basket cells. Taken together, these data provide a direct demonstration that Kvbeta1 and Kvbeta2 associate and colocalize with Kv1 alpha-subunits in native tissues and provide a biochemical and neuroanatomical basis for the differential contribution of Kv1 alpha- and beta-subunits to electrophysiologically diverse neuronal K+ currents.

MeSH Terms
Animals Blotting, Western Brain Chemistry Cerebellum/chemistry Delayed Rectifier Potassium Channels Fluorescent Antibody Technique, Indirect Globus Pallidus/chemistry Immunoenzyme Techniques Interneurons/chemistry Kv1.1 Potassium Channel Kv1.2 Potassium Channel Kv1.4 Potassium Channel Mossy Fibers, Hippocampal/chemistry Nerve Tissue Proteins/analysis,chemistry Organ Specificity Perforant Pathway/chemistry Potassium Channels/analysis,chemistry Potassium Channels, Voltage-Gated Ranvier's Nodes/chemistry Rats Shab Potassium Channels Substantia Nigra/chemistry
Chemicals
Delayed Rectifier Potassium Channels Kcna2 protein, rat Kcna4 protein, rat Kcnb1 protein, rat Kv1.2 Potassium Channel Kv1.4 Potassium Channel Nerve Tissue Proteins Potassium Channels Potassium Channels, Voltage-Gated Shab Potassium Channels Kv1.1 Potassium Channel
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rhodes K J
Central Nervous System Disorders, Wyeth-Ayerst Research, Princeton, New Jersey 08543, USA.
Strassle B W
Monaghan M M
Bekele-Arcuri Z
Matos M F
Trimmer J S
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38 references, click to expand
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-11-01
Pages
8246-58
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6573739
Subset
IM
Grants
NINDS NIH HHS · NS34383 · United States
External Links
PubMed source PMC Full Text View journal details
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