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PMID: 9335582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function.

Genetics ·Vol. 147 ·No. 2 ·1997-10-00 ·Pages 409-20

Kopski KM, Huffaker TC

Abstract

We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene that encodes the 110-kD subunit of the Saccharomyces cerevisiae CBF3 kinetochore complex. At the restrictive temperature of 37 degrees, ndc10-2 cells are able to assemble anaphase spindles, but fail to segregate their DNA, consistent with a defect in kinetochore function. To identify other factors that play a role in kinetochore assembly or function, we isolated both dosage and second site suppressors of the ndc10-2 mutation. These screens identified UBC6 as a dosage suppressor, and mutations in UBC6 and UBC7 as second-site suppressors of ndc10-2 heat sensitivity. Both UBC6 and UBC7 encode ubiquitin-conjugating enzymes that function in ubiquitin-mediated protein degradation. Furthermore, overexpression of a mutant ubiquitin suppresses the ndc10-2 mutation. These results implicate the ubiquitin system in the regulation of ndc10-2 function and suggest a role for the ubiquitin system in kinetochore function.

MeSH Terms
Anaphase-Promoting Complex-Cyclosome Cloning, Molecular DNA-Binding Proteins/genetics Fungal Proteins/genetics Gene Dosage Genes, Lethal Genes, Suppressor Hot Temperature Kinetochores/physiology Ligases/genetics Phenotype Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Ubiquitins/metabolism
Chemicals
CBF2 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Ubiquitins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kopski K M
Section of Biochemistry, Molecular and Cellular Biology, Cornell University, Ithaca, New York 14853-2703, USA.
Huffaker T C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/147.2.409
Published
1997-10-00
Pages
409-20
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208167
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040479 · United States
NIGMS NIH HHS · GM-40479 · United States
External Links
PubMed source DOI: 10.1093/genetics/147.2.409 PMC Full Text View journal details
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