Home LiteratureArticle Details
PMID: 9342322 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning of the cDNA for the TATA-binding protein-associated factorII170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 22 ·1997-10-28 ·Pages 11827-32

van der Knaap JA, Borst JW, van der Vliet PC, Gentz R, Timmers HT

Abstract

The human transcription factor B-TFIID is comprised of TATA-binding protein (TBP) in complex with one TBP-associated factor (TAF) of 170 kDa. We report the isolation of the cDNA for TAFII170. By cofractionation and coprecipitation experiments, we show that the protein encoded by the cDNA encodes the TAF subunit of B-TFIID. Recombinant TAFII170 has (d)ATPase activity. Inspection of its primary structure reveals a striking homology with genes of other organisms, yeast MOT1, and Drosophila moira, which belongs to the Trithorax group. Both homologs were isolated in genetic screens as global regulators of pol II transcription. This supports our classification of B-TFIID as a pol II transcription factor and suggests that specific TBP-TAF complexes perform distinct functions during development.

MeSH Terms
Adenosine Triphosphatases/analysis Amino Acid Sequence Animals Cloning, Molecular DNA, Complementary DNA-Binding Proteins/metabolism Dimerization Drosophila/genetics Humans Molecular Sequence Data Protein Binding RNA Polymerase II Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics Sequence Analysis, DNA TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors/metabolism Transcription Factors, TFII/genetics,metabolism Vaccinia virus/genetics
Chemicals
BTAF1 protein, human DNA, Complementary DNA-Binding Proteins Recombinant Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors Transcription Factors, TFII RNA Polymerase II Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van der Knaap J A
Laboratory for Physiological Chemistry, Utrecht University, P.O. Box 80042, 3508 TA Utrecht, The Netherlands.
Borst J W
van der Vliet P C
Gentz R
Timmers H T
References (34)
34 references, click to expand
  1. Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250.
    Science. 1994 Feb 11;263(5148):811-4 PMID: 8303298
  2. TATA-binding protein and associated factors in polymerase II and polymerase III transcription.
    Mol Cell Biol. 1993 Dec;13(12):7953-60 PMID: 8247010
  3. Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID.
    J Biol Chem. 1994 Sep 16;269(37):23135-40 PMID: 8083216
  4. Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor.
    Cell. 1994 Oct 7;79(1):107-17 PMID: 7923369
  5. Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators.
    Cell. 1994 Oct 7;79(1):93-105 PMID: 7923382
  6. Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.
    Genes Dev. 1994 Aug 15;8(16):1920-34 PMID: 7958867
  7. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  8. Epigenetic effects on yeast transcription caused by mutations in an actin-related protein present in the nucleus.
    Genes Dev. 1996 Mar 1;10(5):604-19 PMID: 8598290
  9. Physical separation of two different forms of human TFIIIB active in the transcription of the U6 or the VAI gene in vitro.
    EMBO J. 1995 Dec 1;14(23):5974-83 PMID: 8846790
  10. Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II.
    Cell. 1995 Jun 30;81(7):1115-25 PMID: 7600579
  11. TAFII250 is a bipartite protein kinase that phosphorylates the base transcription factor RAP74.
    Cell. 1996 Mar 8;84(5):781-90 PMID: 8625415
  12. Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p.
    Mol Cell Biol. 1996 Apr;16(4):1641-8 PMID: 8657139
  13. Transcription activation in cells lacking TAFIIS.
    Nature. 1996 Sep 12;383(6596):185-8 PMID: 8774886
  14. TBP-associated factors are not generally required for transcriptional activation in yeast.
    Nature. 1996 Sep 12;383(6596):188-91 PMID: 8774887
  15. Expanding the Mot1 subfamily: 89B helicase encodes a new Drosophila melanogaster SNF2-related protein which binds to multiple sites on polytene chromosomes.
    Nucleic Acids Res. 1996 Aug 15;24(16):3121-8 PMID: 8774890
  16. Biochemistry and structural biology of transcription factor IID (TFIID).
    Annu Rev Biochem. 1996;65:769-99 PMID: 8811195
  17. Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation.
    Genes Dev. 1996 Sep 15;10(18):2368-80 PMID: 8824595
  18. hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II.
    EMBO J. 1996 Sep 16;15(18):5022-31 PMID: 8890175
  19. The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters.
    Mol Cell Biol. 1996 Dec;16(12):6668-76 PMID: 8943321
  20. Evidence that Spt3 functionally interacts with Mot1, TFIIA, and TATA-binding protein to confer promoter-specific transcriptional control in Saccharomyces cerevisiae.
    Mol Cell Biol. 1997 Jan;17(1):287-95 PMID: 8972209
  21. The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.
    Cell. 1996 Dec 27;87(7):1261-70 PMID: 8980232
  22. SWI2/SNF2 and related proteins: ATP-driven motors that disrupt protein-DNA interactions?
    Cell. 1997 Mar 21;88(6):737-40 PMID: 9118215
  23. c-Myc/Max heterodimers bind cooperatively to the E-box sequences located in the first intron of the rat ornithine decarboxylase (ODC) gene.
    Nucleic Acids Res. 1997 Apr 15;25(8):1493-501 PMID: 9162900
  24. Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA.
    Mol Cell Biol. 1997 Aug;17(8):4842-51 PMID: 9234740
  25. Dosage-dependent modifiers of polycomb and antennapedia mutations in Drosophila.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8136-40 PMID: 3141923
  26. The mammalian TFIID protein is present in two functionally distinct complexes.
    Genes Dev. 1991 Nov;5(11):1946-56 PMID: 1936986
  27. TATA-binding protein is a classless factor.
    Cell. 1992 Mar 6;68(5):819-21 PMID: 1547484
  28. A presumptive helicase (MOT1 gene product) affects gene expression and is required for viability in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Apr;12(4):1879-92 PMID: 1312673
  29. Composition of transcription factor B-TFIID.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8140-4 PMID: 1387711
  30. The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB.
    Cell. 1992 Dec 11;71(6):1015-28 PMID: 1458533
  31. A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.
    Cell. 1992 Dec 11;71(6):1029-40 PMID: 1458534
  32. Control of DNA synthesis genes in budding yeast: involvement of the transcriptional modulator MOT1 in the expression of the DNA polymerase alpha gene.
    Chromosoma. 1992;102(1 Suppl):S107-13 PMID: 1291231
  33. Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.
    Genes Dev. 1993 Jun;7(6):1033-46 PMID: 8504928
  34. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
    Cell. 1994 May 20;77(4):599-608 PMID: 8187178
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.22.11827
Published
1997-10-28
Pages
11827-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23617
Subset
IM
Databases
GENBANK
AJ001017
External Links
PubMed source DOI: 10.1073/pnas.94.22.11827 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2