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PMID: 8774890 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expanding the Mot1 subfamily: 89B helicase encodes a new Drosophila melanogaster SNF2-related protein which binds to multiple sites on polytene chromosomes.

Nucleic acids research ·Vol. 24 ·No. 16 ·1996-08-15 ·Pages 3121-8

Goldman-Levi R, Miller C, Bogoch J, Zak NB

Abstract

Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 family. 89B Helicase protein shows a high degree of homology in its ATPase/helicase domain to the global transcriptional activators SNF2 and Brahma and to the DNA repair proteins ERCC6 and RAD54. It is, however, most strikingly similar to the Saccharomyces cerevisiae protein Mot1, a transcriptional repressor with many target genes for which no homologue has yet been described. 89B helicase is expressed throughout fly development and its large transcript encodes a >200 kDa protein. Staining with anti-89B Helicase antibodies reveals that the protein is present uniformly in early embryos and then becomes localized to the ventral nerve cord and brain. On the polytene chromosomes, 89B Helicase is bound to several hundred specific sites that are randomly distributed. The homology of 89B Helicase to Mot1, its widespread developmental expression and its large number of targets on the polytene chromosomes of larval salivary gland cells suggest that 89B Helicase may play a role in chromosomal metabolism, particularly global transcriptional regulation.

MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Animals Central Nervous System/chemistry Chromosomal Proteins, Non-Histone/classification,genetics,metabolism Chromosomes/chemistry,metabolism,ultrastructure DNA Helicases/classification,genetics,metabolism DNA, Complementary/genetics DNA-Binding Proteins/genetics Drosophila Proteins Drosophila melanogaster/embryology,genetics Genes, Insect Immunohistochemistry Molecular Sequence Data Nuclear Proteins Protein Binding RNA, Messenger/genetics Saccharomyces cerevisiae Proteins Salivary Glands/chemistry Sequence Homology, Amino Acid TATA-Binding Protein Associated Factors Tissue Distribution Transcription Factors/genetics
Chemicals
Chromosomal Proteins, Non-Histone DNA, Complementary DNA-Binding Proteins Drosophila Proteins Nuclear Proteins RNA, Messenger Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors Transcription Factors Adenosine Triphosphatases MOT1 protein, S cerevisiae DNA Helicases Hel89B protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goldman-Levi R
Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Miller C
Bogoch J
Zak N B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.16.3121
Published
1996-08-15
Pages
3121-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146085
Subset
IM
Databases
GENBANK
U45025
External Links
PubMed source DOI: 10.1093/nar/24.16.3121 PMC Full Text View journal details
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