Home LiteratureArticle Details
PMID: 9350770 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.

Journal of clinical microbiology ·Vol. 35 ·No. 11 ·1997-11-00 ·Pages 2969-73

Devallois A, Goh KS, Rastogi N

Abstract

PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was applied to 108 mycobacterial isolates representing 34 species to evaluate its potential as a rapid reference method. A total of 49 distinct patterns were obtained; 25 species were characterized by a single PRA pattern, while 9 species gave more than one specific pattern. An algorithm describing these 34 species (which includes five additional species and new subgroups of Mycobacterium kansasii, M. abscessus, and M. peregrinum) is proposed. A relatively simple and inexpensive method, PRA may be particularly helpful in routine clinical microbiology laboratories.

MeSH Terms
Algorithms Bacterial Proteins/biosynthesis,genetics Chaperonin 60 Chaperonins/biosynthesis,genetics DNA Primers Deoxyribonucleases, Type II Site-Specific Humans Mycobacterium/classification,genetics,isolation & purification Mycobacterium Infections/classification,microbiology Phylogeny Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Reference Standards
Chemicals
Bacterial Proteins Chaperonin 60 DNA Primers heat-shock protein 65, Mycobacterium Deoxyribonucleases, Type II Site-Specific GGCC-specific type II deoxyribonucleases GGTNACC-specific type II deoxyribonucleases Chaperonins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Devallois A
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Point à Pitre, Guadeloupe, French West Indies.
Goh K S
Rastogi N
References (29)
29 references, click to expand
  1. Detection and identification of mycobacteria by amplification of mycobacterial DNA.
    Mol Microbiol. 1989 Jul;3(7):843-9 PMID: 2507865
  2. Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media.
    J Clin Microbiol. 1997 Jan;35(1):79-85 PMID: 8968884
  3. Detection and identification of mycobacteria by amplification of rRNA.
    J Clin Microbiol. 1990 Aug;28(8):1751-9 PMID: 2203812
  4. Characterization of Mycobacterium paratuberculosis and "wood-pigeon" mycobacteria by isoenzyme profile and selective staining of immunoprecipitates.
    Res Microbiol. 1990 Jun;141(5):551-61 PMID: 2218060
  5. Identification of Mycobacterium fortuitum and Mycobacterium chelonei.
    J Clin Microbiol. 1983 May;17(5):744-52 PMID: 6190837
  6. The 65-kilodalton antigen of Mycobacterium tuberculosis.
    J Bacteriol. 1987 Mar;169(3):1080-8 PMID: 3029018
  7. The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein.
    Infect Immun. 1988 Feb;56(2):446-51 PMID: 2892795
  8. Identification of mycobacteria by high-performance liquid chromatography.
    J Clin Microbiol. 1991 Nov;29(11):2468-72 PMID: 1774251
  9. Mycobactin analysis as an aid for the identification of Mycobacterium fortuitum and Mycobacterium chelonae subspecies.
    J Clin Microbiol. 1992 May;30(5):1225-31 PMID: 1583124
  10. Differentiation of slowly growing Mycobacterium species, including Mycobacterium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis.
    J Clin Microbiol. 1992 Jul;30(7):1815-22 PMID: 1352786
  11. Evaluation of nonradioactive DNA probes for identification of mycobacteria.
    J Clin Microbiol. 1992 Sep;30(9):2476-8 PMID: 1401020
  12. Identification of a genetically distinct subspecies of Mycobacterium kansasii.
    J Clin Microbiol. 1992 Nov;30(11):2930-3 PMID: 1280644
  13. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.
    J Clin Microbiol. 1993 Feb;31(2):175-8 PMID: 8381805
  14. Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.
    J Clin Microbiol. 1993 Aug;31(8):2061-5 PMID: 8396586
  15. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.
    J Clin Microbiol. 1993 Nov;31(11):2882-9 PMID: 7505291
  16. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.
    J Clin Microbiol. 1993 Dec;31(12):3216-22 PMID: 7508456
  17. Nucleotide sequence comparison of the mycobacterial dnaJ gene and PCR-restriction fragment length polymorphism analysis for identification of mycobacterial species.
    Int J Syst Bacteriol. 1994 Jan;44(1):159-66 PMID: 7907223
  18. Tentative evidence of AIDS-associated biotype of Mycobacterium kansasii.
    J Clin Microbiol. 1994 Jul;32(7):1779-82 PMID: 7929774
  19. Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing.
    Arch Pathol Lab Med. 1995 Feb;119(2):131-8 PMID: 7848059
  20. Routine identification of Mycobacterium tuberculosis complex isolates by automated hybridization.
    J Clin Microbiol. 1994 Nov;32(11):2702-5 PMID: 7852560
  21. Identification of mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene.
    J Clin Microbiol. 1994 Dec;32(12):2944-7 PMID: 7883881
  22. Demonstration of Mycobacterium kansasii species heterogeneity by the amplification of the 16S-23S spacer region.
    J Med Microbiol. 1995 Aug;43(2):156-8 PMID: 7629856
  23. Evaluation of Amplicor MTB test as adjunct to smears and culture for direct detection of Mycobacterium tuberculosis in the French Caribbean.
    J Clin Microbiol. 1996 May;34(5):1065-8 PMID: 8727876
  24. Molecular technique for rapid identification of mycobacteria.
    J Clin Microbiol. 1996 Jan;34(1):98-102 PMID: 8748282
  25. Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods.
    J Clin Microbiol. 1996 Feb;34(2):296-303 PMID: 8789004
  26. Epidemiology of infection by nontuberculous mycobacteria.
    Clin Microbiol Rev. 1996 Apr;9(2):177-215 PMID: 8964035
  27. Comparative evaluation of PCR and commercial DNA probes for detection and identification to species level of Mycobacterium avium and Mycobacterium intracellulare.
    J Clin Microbiol. 1996 Nov;34(11):2756-9 PMID: 8897178
  28. Genotypic characterization of five subspecies of Mycobacterium kansasii.
    J Clin Microbiol. 1997 Jan;35(1):25-32 PMID: 8968876
  29. DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis.
    J Clin Microbiol. 1989 Dec;27(12):2823-6 PMID: 2574186
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.35.11.2969-2973.1997
Published
1997-11-00
Pages
2969-73
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC230098
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.35.11.2969-2973.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2