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PMID: 9451004 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.

The EMBO journal ·Vol. 17 ·No. 3 ·1998-02-02 ·Pages 797-807

Kiss-László Z, Henry Y, Kiss T

Abstract

Site-specific 2'-O-ribose methylation of eukaryotic rRNAs is guided by small nucleolar RNAs (snoRNAs). The methylation guide snoRNAs carry long perfect complementaries to rRNAs. These antisense elements are located either in the 5' half or in the 3' end region of the snoRNA, and are followed by the conserved D' or D box motifs, respectively. An uninterrupted helix formed between the rRNA and the antisense element of the snoRNA, in conjunction with the adjacent D' or D box, constitute the recognition signal for the putative methyltransferase. Here, we have identified an additional essential box element common to methylation guide snoRNAs, termed the C' box. We show that the C' box functions in concert with the D' box and plays a crucial role in the methyltransfer reaction directed by the upstream antisense element and the D' box. We also show that an internal fragment of U24 methylation guide snoRNA, encompassing the upstream antisense element and the D' and C' box motifs, can support the site-specific methylation of rRNA. This strongly suggests that the C box of methylation guide snoRNAs plays an essential role in the methyltransfer reaction guided by the 3'-terminal antisense element and the D box of the snoRNA.

MeSH Terms
Base Sequence Binding Sites/genetics,physiology Cell Nucleolus/chemistry,metabolism Humans Methylation Molecular Sequence Data Nucleic Acid Conformation RNA Precursors/genetics,metabolism RNA, Antisense/genetics RNA, Guide/analysis,genetics RNA, Ribosomal, 28S/genetics,metabolism RNA, Small Nuclear/analysis,genetics Ribose/metabolism Saccharomyces cerevisiae/chemistry,genetics,physiology Sequence Homology, Nucleic Acid
Chemicals
RNA Precursors RNA, Antisense RNA, Guide RNA, Ribosomal, 28S RNA, Small Nuclear Ribose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kiss-László Z
Laboratoire de Biologie Moléculaire Eucaryote du Centre National de la Recherche Scientifique, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
Henry Y
Kiss T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.3.797
Published
1998-02-02
Pages
797-807
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170428
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.3.797 PMC Full Text View journal details
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