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PMID: 9512523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.

Nucleic acids research ·Vol. 26 ·No. 7 ·1998-04-01 ·Pages 1551-9

Dynan WS, Yoo S

Abstract

The Ku protein-DNA-dependent protein kinase system is one of the major pathways by which cells of higher eukaryotes respond to double-strand DNA breaks. The components of the system are evolutionarily conserved and homologs are known from a number of organisms. The Ku protein component binds directly to DNA ends and may help align them for ligation. Binding of Ku protein to DNA also nucleates formation of an active enzyme complex containing the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). The interaction between Ku protein, DNA-PKcs and nucleic acids has been extensively investigated. This review summarizes the results of these biochemical investigations and relates them to recent molecular genetic studies that reveal highly characteristic repair and recombination defects in mutant cells lacking Ku protein or DNA-PKcs.

MeSH Terms
Amino Acid Sequence Animals Antigens, Nuclear Binding Sites DNA/chemistry,metabolism DNA Damage DNA Helicases DNA Repair DNA-Activated Protein Kinase DNA-Binding Proteins/chemistry,metabolism Ku Autoantigen Macromolecular Substances Mutation Nuclear Proteins/chemistry,metabolism Protein Serine-Threonine Kinases/chemistry,metabolism Recombination, Genetic Sequence Alignment Sequence Homology, Amino Acid Transcription Factors/chemistry,metabolism
Chemicals
Antigens, Nuclear DNA-Binding Proteins Macromolecular Substances Nuclear Proteins Transcription Factors DNA DNA-Activated Protein Kinase Protein Serine-Threonine Kinases DNA Helicases XRCC5 protein, human Xrcc6 protein, human Ku Autoantigen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dynan W S
Program in Gene Regulation, Institute of Molecular Medicine and Genetics, Room CB-2803, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA. [email protected]
Yoo S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.7.1551
Email
[email protected]
Published
1998-04-01
Pages
1551-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147477
Subset
IM
Grants
NIGMS NIH HHS · GM35866 · United States
External Links
PubMed source DOI: 10.1093/nar/26.7.1551 PMC Full Text View journal details
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