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PMID: 9570322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.

RNA (New York, N.Y.) ·Vol. 4 ·No. 2 ·1998-02-00 ·Pages 226-30

Martin G, Keller W

Abstract

We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.

MeSH Terms
Base Sequence Biotin Digoxigenin Molecular Sequence Data Nucleotides/metabolism Polynucleotide Adenylyltransferase/metabolism RNA/chemistry RNA, Catalytic/genetics,metabolism RNA, Messenger/chemistry,metabolism Saccharomyces cerevisiae/enzymology
Chemicals
Nucleotides RNA primers RNA, Catalytic RNA, Messenger RNA Biotin Polynucleotide Adenylyltransferase Digoxigenin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martin G
Department of Cell Biology, Biozentrum, University of Basel, Switzerland.
Keller W
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
Published
1998-02-00
Pages
226-30
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369611
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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