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PMID: 957476 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.

Journal of virology ·Vol. 19 ·No. 2 ·1976-08-00 ·Pages 409-15

Fujisawa H, Hayashi M

Abstract

A DNA protein complex that is a precursor of mature phi X174 phage was isolated. The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA. The viral-strand DNA ranged from one to two genomes in length. Proteins coded on the phi X174 genome as well as the host genome were associated with the viral DNA in the 50S precursor complex. Our results indicated that both viral DNA synthesis and cleavage of the growing viral-strand DNA occurred in the 50S complex.

MeSH Terms
Coliphages/analysis,growth & development,metabolism DNA Replication DNA, Single-Stranded/analysis DNA, Viral/analysis,biosynthesis Morphogenesis Viral Proteins/analysis Virus Replication
Chemicals
DNA, Single-Stranded DNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fujisawa H
Hayashi M
References (33)
33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.19.2.409-415.1976
Published
1976-08-00
Pages
409-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354878
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.19.2.409-415.1976 PMC Full Text View journal details
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