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PMID: 9580676 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analyzing genomes with cumulative skew diagrams.

Nucleic acids research ·Vol. 26 ·No. 10 ·1998-05-15 ·Pages 2286-90

Grigoriev A

Abstract

A novel method of cumulative diagrams shows that the nucleotide composition of a microbial chromosome changes at two points separated by about a half of its length. These points coincide with sites of replication origin and terminus for all bacteria where such sites are known. The leading strand is found to contain more guanine than cytosine residues. This fact is used to predict origin and terminus locations in other bacterial and archaeal genomes. Local changes, visible as diagram distortions, may represent recent genome rearrangements, as demonstrated for two strains of Escherichia coli . Analysis of the diagrams of viral and mitochondrial genomes suggests a link between the base composition bias and the time spent by DNA in a single stranded state during replication.

MeSH Terms
Bacteria/genetics Base Composition Chromosomes, Archaeal Chromosomes, Bacterial DNA Replication DNA, Archaeal/chemistry DNA, Bacterial/chemistry Gene Rearrangement Genome Genome, Bacterial Methanobacterium/genetics Methanococcus/genetics
Chemicals
DNA, Archaeal DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Grigoriev A
Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany. [email protected]
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.10.2286
Email
[email protected]
Published
1998-05-15
Pages
2286-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147580
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/26.10.2286 PMC Full Text View journal details
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