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PMID: 9607915 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hypermutation of immunoglobulin genes in memory B cells of DNA repair-deficient mice.

The Journal of experimental medicine ·Vol. 187 ·No. 11 ·1998-06-01 ·Pages 1735-43

Jacobs H, Fukita Y, van der Horst GT, de Boer J, Weeda G, Essers J, de Wind N, Engelward BP, Samson L, Verbeek S, de Murcia JM, de Murcia G, te Riele H, Rajewsky K

Abstract

To investigate the possible involvement of DNA repair in the process of somatic hypermutation of rearranged immunoglobulin variable (V) region genes, we have analyzed the occurrence, frequency, distribution, and pattern of mutations in rearranged Vlambda1 light chain genes from naive and memory B cells in DNA repair-deficient mutant mouse strains. Hypermutation was found unaffected in mice carrying mutations in either of the following DNA repair genes: xeroderma pigmentosum complementation group (XP)A and XPD, Cockayne syndrome complementation group B (CSB), mutS homologue 2 (MSH2), radiation sensitivity 54 (RAD54), poly (ADP-ribose) polymerase (PARP), and 3-alkyladenine DNA-glycosylase (AAG). These results indicate that both subpathways of nucleotide excision repair, global genome repair, and transcription-coupled repair are not required for somatic hypermutation. This appears also to be true for mismatch repair, RAD54-dependent double-strand-break repair, and AAG-mediated base excision repair.

MeSH Terms
Animals B-Lymphocytes/immunology DNA Repair/physiology Gene Rearrangement, B-Lymphocyte Genes, Immunoglobulin Immunoglobulin Variable Region/genetics Immunoglobulin lambda-Chains/genetics Immunologic Memory/immunology Mice Mice, Mutant Strains Mutation Polymerase Chain Reaction
Chemicals
Immunoglobulin Variable Region Immunoglobulin lambda-Chains
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Jacobs H
Basel Institute for Immunology, CH-4005 Basel, Switzerland. [email protected]
Fukita Y
van der Horst G T
de Boer J
Weeda G
Essers J
de Wind N
Engelward B P
Samson L
Verbeek S
de Murcia J M
de Murcia G
te Riele H
Rajewsky K
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.187.11.1735
Email
[email protected]
Published
1998-06-01
Pages
1735-43
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2212309
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.187.11.1735 PMC Full Text View journal details
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