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PMID: 9632587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Salmonella typhimurium AhpC polypeptide is not essential for virulence in BALB/c mice but is recognized as an antigen during infection.

Infection and immunity ·Vol. 66 ·No. 7 ·1998-07-00 ·Pages 3208-17

Taylor PD, Inchley CJ, Gallagher MP

Abstract

The OxyR regulon is known to mediate protection against oxidizing agents in Salmonella typhimurium. We reported previously that ahp, one of the OxyR-regulated loci, is induced during macrophage interaction (K. P. Francis, P. D. Taylor, C. J. Inchley, and M. P. Gallagher, J. Bacteriol. 179:4046-4048, 1997). We now report on the effects of disrupting ahp or oxyR on virulence in a BALB/c mouse model. Surprisingly, insertion of a Mudlux derivative within ahpC was found to result in attenuation, while irreversible inactivation of the locus through insertion of a cml cassette did not. An SL1344 derivative carrying an oxyR::kan disruption was also found to be as virulent as the parental strain. Moreover, both cell-mediated and humoral responses to AhpC were found to develop during the course of infection, probably through T-helper-cell (type I) activation. These results indicate that, although not essential for virulence, AhpC is expressed by S. typhimurium during infection of BALB/c mice and constitutes a target for the immune system.

MeSH Terms
Animals Antigens, Bacterial/physiology DNA-Binding Proteins Female Immunologic Memory Mice Mice, Inbred BALB C Oxidoreductases/physiology Peroxidases Peroxiredoxins Repressor Proteins/physiology Salmonella Infections, Animal/immunology Salmonella typhimurium/immunology,pathogenicity T-Lymphocytes/immunology Transcription Factors/physiology Virulence
Chemicals
Antigens, Bacterial DNA-Binding Proteins Repressor Proteins Transcription Factors Oxidoreductases Peroxidases Peroxiredoxins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Taylor P D
Division of Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kingdom.
Inchley C J
Gallagher M P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.7.3208-3217.1998
Published
1998-07-00
Pages
3208-17
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108334
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.66.7.3208-3217.1998 PMC Full Text View journal details
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