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PMID: 9707531 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes.

The Plant cell ·Vol. 10 ·No. 8 ·1998-08-00 ·Pages 1307-19

Milligan SB, Bodeau J, Yaghoobi J, Kaloshian I, Zabel P, Williamson VM

Abstract

The Mi locus of tomato confers resistance to root knot nematodes. Tomato DNA spanning the locus was isolated as bacterial artificial chromosome clones, and 52 kb of contiguous DNA was sequenced. Three open reading frames were identified with similarity to cloned plant disease resistance genes. Two of them, Mi-1.1 and Mi-1.2, appear to be intact genes; the third is a pseudogene. A 4-kb mRNA hybridizing with these genes is present in tomato roots. Complementation studies using cloned copies of Mi-1.1 and Mi-1.2 indicated that Mi-1.2, but not Mi-1.1, is sufficient to confer resistance to a susceptible tomato line with the progeny of transformants segregating for resistance. The cloned gene most similar to Mi-1.2 is Prf, a tomato gene required for resistance to Pseudomonas syringae. Prf and Mi-1.2 share several structural motifs, including a nucleotide binding site and a leucine-rich repeat region, that are characteristic of a family of plant proteins, including several that are required for resistance against viruses, bacteria, fungi, and now, nematodes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Primers DNA-Binding Proteins/genetics Genes, Plant Leucine/chemistry Leucine Zippers/genetics Lycopersicon esculentum/genetics Microphthalmia-Associated Transcription Factor Molecular Sequence Data Nematoda/pathogenicity Nucleotides/metabolism Plants, Genetically Modified Sequence Homology, Amino Acid Transcription Factors Transcription, Genetic
Chemicals
DNA Primers DNA-Binding Proteins Microphthalmia-Associated Transcription Factor Nucleotides Transcription Factors Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Milligan S B
Center for Engineering Plants for Resistance Against Pathogens, University of California, Davis, California 95616, USA.
Bodeau J
Yaghoobi J
Kaloshian I
Zabel P
Williamson V M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.8.1307
Published
1998-08-00
Pages
1307-19
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144378
Subset
IM
Databases
GENBANK
AF039681, AF039682
External Links
PubMed source DOI: 10.1105/tpc.10.8.1307 PMC Full Text View journal details
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