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PMID: 9724648 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active recruitment of sigma54-RNA polymerase to the Pu promoter of Pseudomonas putida: role of IHF and alphaCTD.

The EMBO journal ·Vol. 17 ·No. 17 ·1998-09-01 ·Pages 5120-8

Bertoni G, Fujita N, Ishihama A, de Lorenzo V

Abstract

The sequence elements determining the binding of the sigma54-containing RNA polymerase (sigma54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to previous results in related systems, we show that the integration host factor (IHF) binding stimulates the recruitment of the enzyme to the -12/-24 sequence motifs. Such a recruitment, which is fully independent of the activator of the system, XylR, requires the interaction of the C-terminal domain of the alpha subunit of RNAP with specific DNA sequences upstream of the IHF site which are reminiscent of the UP elements in sigma70 promoters. Our data show that this interaction is mainly brought about by the distinct geometry of the promoter region caused by IHF binding and the ensuing DNA bending. These results support the view that binding of sigma54-RNAP to a promoter is a step that can be subjected to regulation by factors (e.g. IHF) other than the sole intrinsic affinity of sigma54-RNAP for the -12/-24 site.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Binding Sites DNA Footprinting DNA-Binding Proteins DNA-Directed RNA Polymerases/metabolism Integration Host Factors Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Peptide Fragments/metabolism Promoter Regions, Genetic Protein Binding Pseudomonas putida/genetics RNA Polymerase Sigma 54 Sigma Factor/metabolism
Chemicals
Bacterial Proteins DNA-Binding Proteins Integration Host Factors Peptide Fragments Sigma Factor integration host factor, Pseudomonas DNA-Directed RNA Polymerases RNA Polymerase Sigma 54
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bertoni G
Department of Microbial Biotechnology, Centro Nacional de Biotecnología CSIC, Campus de Cantoblanco, 28049 Madrid, Spain.
Fujita N
Ishihama A
de Lorenzo V
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.17.5120
Published
1998-09-01
Pages
5120-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170840
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.17.5120 PMC Full Text View journal details
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