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PMID: 9725831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage.

Genetics ·Vol. 150 ·No. 1 ·1998-09-00 ·Pages 75-93

Paulovich AG, Armour CD, Hartwell LH

Abstract

In wild-type Saccharomyces cerevisiae, a checkpoint slows the rate of progression of an ongoing S phase in response to exposure to a DNA-alkylating agent. Mutations that eliminate S phase regulation also confer sensitivity to alkylating agents, leading us to suggest that, by regulating the S phase rate, cells are either better able to repair or better able to replicate damaged DNA. In this study, we determine the effects of mutations that impair S phase regulation on the ability of excision repair-defective cells to replicate irreparably UV-damaged DNA. We assay survival after UV irradiation, as well as the genetic consequences of replicating a damaged template, namely mutation and sister chromatid exchange induction. We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 (but not REV3, RAD24, and MEC3) are required for maximal induction of replication-dependent sister chromatid exchange. Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication.

MeSH Terms
Adaptation, Physiological/genetics Base Sequence DNA Damage DNA Primers DNA Repair/genetics Genes, Fungal Molecular Sequence Data Mutagenesis Nucleic Acid Heteroduplexes Saccharomyces cerevisiae/genetics,physiology,radiation effects Sister Chromatid Exchange Ultraviolet Rays
Chemicals
DNA Primers Nucleic Acid Heteroduplexes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Paulovich A G
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Armour C D
Hartwell L H
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/150.1.75
Published
1998-09-00
Pages
75-93
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460327
Subset
IM
Grants
NIGMS NIH HHS · GM-17709 · United States
External Links
PubMed source DOI: 10.1093/genetics/150.1.75 PMC Full Text View journal details
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