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PMID: 97263 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Post-transcriptional modifications of the anticodon loop region: alterations in isoaccepting species of tRNA's during development in Bacillus subtilis.

Journal of bacteriology ·Vol. 135 ·No. 1 ·1978-07-00 ·Pages 124-32

Vold BS

Abstract

Structural similarities of tRNA's were compared using three sets of isoaccepting species that had previously been shown to undergo significant changes in chromatographic elution properties as a function of developmental stage in Bacillus subtilis. Comparisons of the structures of the tRNA's were based on the composition of their modified nucleosides, comparisons of oligonucleotide elution profiles from RPC-5 columns, and two-dimensional electrophoretic fingerprint analysis of oligonucleotides. The tRNA's studied were tRNA(Lys) (1) and tRNA(Lys) (3); tRNA(Tyr) (1) and tRNA(Tyr) (2); and tRNA(Trp) (1) and tRNA(Trp) (2). The results suggest that the difference among these pairs of isoaccepting species is a difference in the degree of post-transcriptional modifications of the anticodon loop region. The nucleosides involved were N(6)-(Delta(2)-isopentenyl)adenosine (i(6)A), 2-methylthio-N(6)-(Delta(2)-isopentenyl)adenosine (ms(2)i(6)A), and an unknown nucleoside K, which occurred in a position analogous to N-[9-(beta-d-ribofuranosyl)purin-6-ylcarbamoyl]threonine. The amounts of i(6)A and ms(2)i(6)A, determined using total tRNA from exponential-or stationary-phase cells, suggest that the thiomethylation of i(6)A is a pleiotropic phenomenon affecting several tRNA species. As opposed to the situation in Escherichia coli tRNA, where ms(2)i(6)A constitutes about 90% of the total hydrophobic nucleosides at all growth stages, B. subtilis tRNA's have i(6)A as the predominant hydrophobic nucleoside in exponential growth and ms(2)i(6)A as the predominant nucleoside in stationary phase. Thus, the enzyme system which forms i(6)A and the enzyme system which thiomethylates i(6)A are not coordinated during growth in B. subtilis as they are in E. coli. It is suggested that these changes in anticodon loop modifications in B. subtilis may be related to changes in the translational apparatus which occur during sporulation.

MeSH Terms
Bacillus subtilis/analysis,genetics,growth & development Nucleic Acid Conformation Nucleosides/analysis Oligonucleotides/analysis RNA, Bacterial/analysis RNA, Transfer/analysis Spores, Bacterial/growth & development Transcription, Genetic
Chemicals
Nucleosides Oligonucleotides RNA, Bacterial RNA, Transfer
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Vold B S
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.135.1.124-132.1978
Published
1978-07-00
Pages
124-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC224786
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.135.1.124-132.1978 PMC Full Text View journal details
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