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PMID: 9774671 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 18 ·No. 11 ·1998-11-00 ·Pages 6560-70

Karpichev IV, Small GM

Abstract

Two transcription factors, Oaf1p and Pip2p (Oaf2p), are key components in the pathway by which several Saccharomyces cerevisiae genes encoding peroxisomal proteins are activated in the presence of a fatty acid such as oleate. By searching the S. cerevisiae genomic database for the consensus sequence that acts as a target for these transcription factors, we identified 40 genes that contain a putative Oaf1p-Pip2p binding site in their promoter region. Quantitative Northern analysis confirmed that the expression of 22 of the genes identified is induced by oleate and that either one or both of these transcription factors are required for the activation. In addition to known peroxisomal proteins, the regulated genes encode novel peroxisomal proteins, a mitochondrial protein, and proteins of unknown location and function. We demonstrate that Oaf1p regulates certain genes in the absence of Pip2p and that both of these transcription factors play a role in maintaining the glucose-repressed state of one gene. Furthermore, we provide evidence that the defined consensus binding site is not required for the regulation of certain oleate-responsive genes.

MeSH Terms
ATP-Binding Cassette Transporters/genetics Binding Sites/genetics Catalase/genetics Citrate (si)-Synthase/genetics Consensus Sequence/genetics DNA-Binding Proteins/genetics Fungal Proteins/physiology Gene Expression Regulation, Enzymologic/genetics Gene Expression Regulation, Fungal/genetics Genes, Fungal/drug effects,genetics Microbodies/metabolism Oleic Acid/genetics,pharmacology RNA, Messenger/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins Transcription Factors/physiology
Chemicals
ATP-Binding Cassette Transporters DNA-Binding Proteins Fungal Proteins PXA1 protein, S cerevisiae RNA, Messenger Saccharomyces cerevisiae Proteins Transcription Factors Oleic Acid Catalase Citrate (si)-Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karpichev I V
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029, USA.
Small G M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.11.6560
Published
1998-11-00
Pages
6560-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109241
Subset
IM
Grants
NIDDK NIH HHS · R55DKOD51992 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.11.6560 PMC Full Text View journal details
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