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PMID: 9822592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Localization of the 26S proteasome during mitosis and meiosis in fission yeast.

The EMBO journal ·Vol. 17 ·No. 22 ·1998-11-16 ·Pages 6465-76

Wilkinson CR, Wallace M, Morphew M, Perry P, Allshire R, Javerzat JP, McIntosh JR, Gordon C

Abstract

The 26S proteasome is a large multisubunit complex involved in degrading both cytoplasmic and nuclear proteins. We have investigated the localization of this complex in the fission yeast, Schizosaccharomyces pombe. Immunofluorescence microscopy shows a striking localization pattern whereby the proteasome is found predominantly at the nuclear periphery, both in interphase and throughout mitosis. Electron microscopic analysis revealed a concentration of label near the inner side of the nuclear envelope. The localization of green fluorescent protein (GFP)-tagged 26S proteasomes was analyzed in live cells during mitosis and meiosis. Throughout mitosis the proteasome remained predominantly at the nuclear periphery. During meiosis the proteasome was found to undergo dramatic changes in its localization. Throughout the first meiotic division, the signal is more dispersed over the nucleus. During meiosis II, there was a dramatic re-localization, and the signal became restricted to the area between the separating DNA until the end of meiosis when the signal dispersed before returning to the nuclear periphery during spore formation. These findings strongly imply that the nuclear periphery is a major site of protein degradation in fission yeast both in interphase and throughout mitosis. Furthermore they raise interesting questions as to the spatial organization of protein degradation during meiosis.

MeSH Terms
Base Sequence Carrier Proteins/metabolism Cysteine Endopeptidases/metabolism DNA Primers Immunohistochemistry Intracellular Signaling Peptides and Proteins Meiosis Microscopy, Electron Mitosis Multienzyme Complexes/metabolism Nuclear Envelope/enzymology Proteasome Endopeptidase Complex Schizosaccharomyces/cytology,enzymology,ultrastructure Schizosaccharomyces pombe Proteins Trans-Activators/metabolism
Chemicals
Carrier Proteins DNA Primers Intracellular Signaling Peptides and Proteins MTS4 protein, S pombe Multienzyme Complexes Schizosaccharomyces pombe Proteins Trans-Activators rpn11 protein, S pombe Cysteine Endopeptidases Proteasome Endopeptidase Complex
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wilkinson C R
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK.
Wallace M
Morphew M
Perry P
Allshire R
Javerzat J P
McIntosh J R
Gordon C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.22.6465
Published
1998-11-16
Pages
6465-76
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170994
Subset
IM
Grants
NCRR NIH HHS · RR00592 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.22.6465 PMC Full Text View journal details
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