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PMID: 9843582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional characterization of a Nup159p-containing nuclear pore subcomplex.

Molecular biology of the cell ·Vol. 9 ·No. 12 ·1998-12-00 ·Pages 3475-92

Belgareh N, Snay-Hodge C, Pasteau F, Dagher S, Cole CN, Doye V

Abstract

Nup159p/Rat7p is an essential FG repeat-containing nucleoporin localized at the cytoplasmic face of the nuclear pore complex (NPC) and involved in poly(A)+ RNA export and NPC distribution. A detailed structural-functional analysis of this nucleoporin previously demonstrated that Nup159p is anchored within the NPC through its essential carboxyl-terminal domain. In this study, we demonstrate that Nup159p specifically interacts through this domain with both Nsp1p and Nup82p. Further analysis of the interactions within the Nup159p/Nsp1p/Nup82p subcomplex using the nup82Delta108 mutant strain revealed that a deletion within the carboxyl-terminal domain of Nup82p prevents its interaction with Nsp1p but does not affect the interaction between Nup159p and Nsp1p. Moreover, immunofluorescence analysis demonstrated that Nup159p is delocalized from the NPC in nup82Delta108 cells grown at 37 degrees C, a temperature at which the Nup82Delta108p mutant protein becomes degraded. This suggests that Nup82p may act as a docking site for a core complex composed of the repeat-containing nucleoporins Nup159p and Nsp1p. In vivo transport assays further revealed that nup82Delta108 and nup159-1/rat7-1 mutant strains have little if any defect in nuclear protein import and protein export. Together our data suggest that the poly(A)+ RNA export defect previously observed in nup82 mutant cells might be due to the loss from the NPCs of the repeat-containing nucleoporin Nup159p.

MeSH Terms
Biological Transport, Active Calcium-Binding Proteins Fungal Proteins/chemistry,genetics,metabolism Macromolecular Substances Membrane Proteins/chemistry,genetics,metabolism Microscopy, Electron Mutation Nuclear Envelope/metabolism,ultrastructure Nuclear Pore Complex Proteins Nuclear Proteins/chemistry,genetics,metabolism Plasmids/genetics Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins
Chemicals
Calcium-Binding Proteins Fungal Proteins Macromolecular Substances Membrane Proteins NIC96 protein, S cerevisiae NSP1 protein, S cerevisiae NUP159 protein, S cerevisiae NUP82 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Belgareh N
Centre National de la Recherche Scientifique, UMR144, Institut Curie, 75 248 Paris cedex 05, France.
Snay-Hodge C
Pasteau F
Dagher S
Cole C N
Doye V
References (56)
56 references, click to expand
  1. Regulated nuclear translocation of the Mig1 glucose repressor.
    Mol Biol Cell. 1997 Aug;8(8):1603-18 PMID: 9285828
  2. In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p.
    Mol Biol Cell. 1997 Jan;8(1):33-46 PMID: 9017593
  3. Exportin 1 (Crm1p) is an essential nuclear export factor.
    Cell. 1997 Sep 19;90(6):1041-50 PMID: 9323132
  4. Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
    Mol Biol Cell. 1997 Oct;8(10):2017-38 PMID: 9348540
  5. A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae.
    J Cell Sci. 1997 Dec;110 ( Pt 23):2987-99 PMID: 9359887
  6. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.
    Curr Biol. 1997 Oct 1;7(10):767-75 PMID: 9368759
  7. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.
    J Cell Biol. 1997 Dec 29;139(7):1645-53 PMID: 9412460
  8. A nuclear import pathway for a protein involved in tRNA maturation.
    J Cell Biol. 1997 Dec 29;139(7):1655-61 PMID: 9412461
  9. Nucleocytoplasmic transport: the last 200 nanometers.
    Cell. 1998 Feb 6;92(3):327-36 PMID: 9476893
  10. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p.
    EMBO J. 1998 Apr 15;17(8):2196-207 PMID: 9545233
  11. Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex.
    Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11241-5 PMID: 9736720
  12. Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes.
    J Cell Biol. 1998 Nov 2;143(3):577-88 PMID: 9813081
  13. Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae.
    J Bacteriol. 1975 Oct;124(1):511-23 PMID: 1100612
  14. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  15. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein.
    J Cell Biol. 1988 Jul;107(1):17-31 PMID: 3292539
  16. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  17. Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments.
    J Cell Biol. 1997 Feb 10;136(3):515-29 PMID: 9024684
  18. Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells.
    J Cell Biol. 1997 Feb 24;136(4):747-59 PMID: 9049242
  19. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
    EMBO J. 1997 Feb 17;16(4):807-16 PMID: 9049309
  20. Nucleocytoplasmic transport: signals, mechanisms and regulation.
    Nature. 1997 Apr 24;386(6627):779-87 PMID: 9126736
  21. Cloning and characterization of human karyopherin beta3.
    Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4451-6 PMID: 9114010
  22. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p.
    J Cell Biol. 1997 May 19;137(4):797-811 PMID: 9151683
  23. From nucleoporins to nuclear pore complexes.
    Curr Opin Cell Biol. 1997 Jun;9(3):401-11 PMID: 9159086
  24. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex.
    J Cell Biol. 1997 Jun 2;137(5):989-1000 PMID: 9166401
  25. Nucleocytoplasmic transport of macromolecules.
    Microbiol Mol Biol Rev. 1997 Jun;61(2):193-211 PMID: 9184010
  26. Transmission electron microscopy and immunocytochemical studies of yeast: analysis of HMG-CoA reductase overproduction by electron microscopy.
    Methods Cell Biol. 1989;31:473-512 PMID: 2674630
  27. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain.
    Cell. 1990 Jun 15;61(6):979-89 PMID: 2112428
  28. A complex of nuclear pore proteins required for pore function.
    J Cell Biol. 1991 Jul;114(1):169-83 PMID: 2050741
  29. A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.
    EMBO J. 1992 Dec;11(13):5051-61 PMID: 1464327
  30. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation.
    Eur J Cell Biol. 1992 Dec;59(2):280-95 PMID: 1493793
  31. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96.
    EMBO J. 1993 Aug;12(8):3061-71 PMID: 7688296
  32. Isolation of the yeast nuclear pore complex.
    J Cell Biol. 1993 Nov;123(4):771-83 PMID: 8227139
  33. Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1.
    Eur J Cell Biol. 1993 Oct;62(1):1-12 PMID: 8269967
  34. The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1519-23 PMID: 8108440
  35. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure.
    J Cell Biol. 1994 Jun;125(5):955-69 PMID: 8195299
  36. Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification.
    EMBO J. 1994 Jul 1;13(13):3136-48 PMID: 8039506
  37. Differential mitotic phosphorylation of proteins of the nuclear pore complex.
    J Biol Chem. 1995 Jan 6;270(1):254-62 PMID: 7814383
  38. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
    EMBO J. 1995 Jan 3;14(1):76-87 PMID: 7828598
  39. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
    J Cell Biol. 1995 May;129(4):939-55 PMID: 7744966
  40. Relocation of the carboxyterminal part of CAN from the nuclear envelope to the nucleus as a result of leukemia-specific chromosome rearrangements.
    Oncogene. 1995 May 4;10(9):1739-48 PMID: 7753551
  41. The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate.
    J Biol Chem. 1995 Aug 11;270(32):19017-21 PMID: 7642562
  42. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p.
    J Cell Biol. 1995 Sep;130(6):1263-73 PMID: 7559750
  43. NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export.
    J Cell Biol. 1995 Sep;130(6):1275-81 PMID: 7559751
  44. Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
    J Cell Biol. 1995 Dec;131(5):1133-48 PMID: 8522578
  45. Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1659-75 PMID: 8557736
  46. Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1677-97 PMID: 8557737
  47. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
    Cell. 1996 Jan 26;84(2):265-75 PMID: 8565072
  48. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.
    Mol Biol Cell. 1995 Nov;6(11):1591-603 PMID: 8589458
  49. The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex.
    J Cell Biol. 1996 Jun;133(6):1153-62 PMID: 8682855
  50. Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins.
    J Cell Biol. 1996 Aug;134(3):589-601 PMID: 8707840
  51. Toward the molecular dissection of protein import into nuclei.
    Curr Opin Cell Biol. 1996 Jun;8(3):397-406 PMID: 8743893
  52. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p.
    Mol Biol Cell. 1996 Jun;7(6):917-34 PMID: 8816998
  53. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
    Science. 1996 Oct 25;274(5287):624-7 PMID: 8849456
  54. Interaction of cellular proteins with the leukemia specific fusion proteins DEK-CAN and SET-CAN and their normal counterpart, the nucleoporin CAN.
    Oncogene. 1996 Oct 17;13(8):1801-8 PMID: 8895527
  55. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation.
    J Cell Biol. 1996 Oct;135(2):329-39 PMID: 8896592
  56. Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins.
    EMBO J. 1997 Aug 15;16(16):5086-97 PMID: 9305650
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.12.3475
Published
1998-12-00
Pages
3475-92
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25658
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033998 · United States
NIGMS NIH HHS · GM33998 · United States
NIAMS NIH HHS · AR07576 · United States
External Links
PubMed source DOI: 10.1091/mbc.9.12.3475 PMC Full Text View journal details
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