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PMID: 7828598 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.

The EMBO journal ·Vol. 14 ·No. 1 ·1995-01-03 ·Pages 76-87

Grandi P, Schlaich N, Tekotte H, Hurt EC

Abstract

Nic96p has been isolated previously in a complex together with the nuclear pore proteins Nsp1p, Nup49p and a p54 polypeptide. In a genetic screen for Nsp1p-interacting components, we now find NIC96, as well as a novel gene NUP57 which encodes the p54 protein (called Nup57p). Nup57p which is essential for cell growth contains GLFG repeats in the N-terminal half and heptad repeats in the C-terminal half. The domain organization of Nic96p is more complex: N-terminally located heptad repeats mediate binding to a trimeric Nsp1p-Nup49p-Nup57p complex, but are not required for the formation of this core complex; single amino acid substitutions in the central domain yield thermosensitive mutants, which do not impair interaction with the Nsp1 complex; the C-terminal domain is neither essential nor required for binding to the nucleoporin complex, but strikingly mutations in this part cause synthetic lethality with nsp1 and nup57 mutant alleles. Since a strain in which the Nic96p heptad repeats were deleted shows, similar to nsp1 and nup49 mutants, cytoplasmic mislocalization of a nuclear reporter protein, we propose that the interaction of the heterotrimeric Nsp1p-Nup49p-Nup57p core complex with Nic96p is required for protein transport into the nucleus.

Related Genes
NIC96 NUP57
MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport/genetics Calcium-Binding Proteins Cell Compartmentation Cell Nucleus/metabolism Cytoplasm/metabolism DNA Mutational Analysis Fungal Proteins/genetics,metabolism Genes, Fungal/genetics Genes, Lethal/genetics Genetic Complementation Test Macromolecular Substances Membrane Proteins/genetics,metabolism Molecular Sequence Data Nuclear Envelope/genetics,metabolism Nuclear Pore Complex Proteins Nuclear Proteins/genetics,metabolism Protein Binding Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae Proteins Selection, Genetic Structure-Activity Relationship Yeasts
Chemicals
Calcium-Binding Proteins Fungal Proteins Macromolecular Substances Membrane Proteins NIC96 protein, S cerevisiae NSP1 protein, S cerevisiae NUP49 protein, S cerevisiae NUP57 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grandi P
EMBL, Heidelberg, Germany.
Schlaich N
Tekotte H
Hurt E C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-01-03
Pages
76-87
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398054
Subset
IM
Databases
GENBANK
X81155
External Links
PubMed source PMC Full Text View journal details
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