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PMID: 9847314 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev.

Journal of virology ·Vol. 73 ·No. 1 ·1999-01-00 ·Pages 120-7

Zolotukhin AS, Felber BK

Abstract

Human immunodeficiency virus type 1 (HIV-1) Rev contains a leucine-rich nuclear export signal that is essential for its nucleocytoplasmic export mediated by hCRM1. We examined the role of selected nucleoporins, which are located in peripheral structures of the nuclear pore complex and are thought to be involved in export, in Rev function in human cells. First, we found that upon actinomycin D treatment, Nup98, but not Nup214 or Nup153, is able to translocate to the cytoplasm of HeLa cells, demonstrating that Nup98 may act as a soluble factor. We further showed that Rev can recruit Nup98 and Nup214, but not Nup153, to the nucleolus. We also found that the isolated FG-containing repeat domains of Nup98 and Nup214, but not those of Nup153, competitively inhibit the Rev/RRE-mediated expression of HIV. Taken together, the recruitment of Nup98 and Nup214 by Rev and the competitive inhibition exhibited by their NP domains demonstrate direct participation of Nup98 and Nup214 in the Rev-hCRM1-mediated export.

MeSH Terms
Animals Biological Transport Carrier Proteins/physiology Cell Nucleolus/metabolism Cell Nucleus/metabolism Dactinomycin/pharmacology Gene Products, rev/metabolism HIV-1/physiology HeLa Cells Humans Karyopherins Nuclear Pore Complex Proteins Nuclear Proteins/physiology Receptors, Cytoplasmic and Nuclear Repetitive Sequences, Amino Acid Xenopus rev Gene Products, Human Immunodeficiency Virus
Chemicals
Carrier Proteins Gene Products, rev Karyopherins NUP153 protein, human Nuclear Pore Complex Proteins Nuclear Proteins Receptors, Cytoplasmic and Nuclear exportin 1 protein rev Gene Products, Human Immunodeficiency Virus Dactinomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zolotukhin A S
Human Retrovirus Pathogenesis Group, ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
Felber B K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.1.120-127.1999
Published
1999-01-00
Pages
120-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC103815
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.1.120-127.1999 PMC Full Text View journal details
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