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PMID: 9869632 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

c-myc null cells misregulate cad and gadd45 but not other proposed c-Myc targets.

Genes & development ·Vol. 12 ·No. 24 ·1998-12-15 ·Pages 3797-802

Bush A, Mateyak M, Dugan K, Obaya A, Adachi S, Sedivy J, Cole M

Abstract

We report here that the expression of virtually all proposed c-Myc target genes is unchanged in cells containing a homozygous null deletion of c-myc. Two noteworthy exceptions are the gene cad, which has reduced log phase expression and serum induction in c-myc null cells, and the growth arrest gene gadd45, which is derepressed by c-myc knockout. Thus, cad and gadd45 are the only proposed targets of c-Myc that may contribute to the dramatic slow growth phenotype of c-myc null cells. Our results demonstrate that a loss-of-function approach is critical for the evaluation of potential c-Myc target genes.

MeSH Terms
Animals Aspartate Carbamoyltransferase/genetics,metabolism Blotting, Northern Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics,metabolism Cell Cycle Cell Division Cell Line Dihydroorotase/genetics,metabolism Down-Regulation Eukaryotic Initiation Factor-4E Gene Deletion Gene Expression Regulation Genes, myc Genes, p53 Homozygote Intracellular Signaling Peptides and Proteins Multienzyme Complexes/genetics,metabolism Ornithine Decarboxylase/genetics Peptide Initiation Factors/genetics Phenotype Protein Tyrosine Phosphatases/genetics Proteins/genetics,metabolism Proto-Oncogene Proteins c-myc/physiology Rats cdc25 Phosphatases
Chemicals
CAD trifunctional enzyme Eukaryotic Initiation Factor-4E GADD45 protein Intracellular Signaling Peptides and Proteins Multienzyme Complexes Peptide Initiation Factors Proteins Proto-Oncogene Proteins c-myc Aspartate Carbamoyltransferase Cdc25a protein, rat Protein Tyrosine Phosphatases cdc25 Phosphatases Dihydroorotase Ornithine Decarboxylase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bush A
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 USA.
Mateyak M
Dugan K
Obaya A
Adachi S
Sedivy J
Cole M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.24.3797
Published
1998-12-15
Pages
3797-802
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317273
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.24.3797 PMC Full Text View journal details
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