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PMID: 9892607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The cutaneous lymphocyte antigen is an essential component of the L-selectin ligand induced on human vascular endothelial cells.

The Journal of experimental medicine ·Vol. 189 ·No. 2 ·1999-01-18 ·Pages 241-52

Tu L, Delahunty MD, Ding H, Luscinskas FW, Tedder TF

Abstract

L-selectin mediates leukocyte rolling on vascular endothelium during inflammation. Although vascular endothelium can be activated with inflammatory cytokines to express functional L-selectin ligands, these ligands have not been well characterized. In this study, fucosyltransferase VII cDNA (Fuc-TVII) transfection of the EA.hy926 human vascular endothelial cell line (926-FtVII) induced functional L-selectin ligand expression and expression of sialyl Lewisx (sLex), as defined by HECA-452 (cutaneous lymphocyte antigen; CLA) and CSLEX-1 mAbs. Cytokine activation of human umbilical vein endothelial cells (HUVEC) also induced functional L-selectin ligand expression, with increased CLA expression and Fuc-TVII transcription. The majority of L-selectin-dependent lymphocyte attachment to activated HUVEC and 926-FtVII cells was blocked specifically by treating the endothelial cells with the HECA-452 mAb, but not the CSLEX-1 mAb. CLA-bearing ligands on vascular endothelium also required sulfation and appropriate molecular scaffolds for functional activity, but were distinct from the L-selectin ligands previously identified by the MECA-79 mAb. These findings demonstrate that the HECA-452- defined antigen, CLA, is an essential carbohydrate component of vascular L-selectin ligands.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte Antigens, Neoplasm Cell Adhesion/immunology Cell Line Endothelium, Vascular/immunology Fluorescent Antibody Technique Fucosyltransferases/genetics Humans Immunoglobulin M/genetics,immunology Inflammation/immunology L-Selectin/immunology Leukocytes/immunology Ligands Lymphocytes/metabolism Membrane Glycoproteins/immunology Oligosaccharides/immunology Recombinant Fusion Proteins/genetics Sialyl Lewis X Antigen Transfection/genetics Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Neoplasm CTAGE1 protein, human Immunoglobulin M Ligands Membrane Glycoproteins Oligosaccharides Recombinant Fusion Proteins Sialyl Lewis X Antigen Tumor Necrosis Factor-alpha L-Selectin Fucosyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tu L
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Delahunty M D
Ding H
Luscinskas F W
Tedder T F
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.189.2.241
Published
1999-01-18
Pages
241-52
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192992
Subset
IM
Grants
NCI NIH HHS · CA-54464 · United States
NIAID NIH HHS · AI-26872 · United States
NHLBI NIH HHS · HL-50985 · United States
External Links
PubMed source DOI: 10.1084/jem.189.2.241 PMC Full Text View journal details
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