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PMID: 9973359 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Conversion of 3-chlorocatechol by various catechol 2,3-dioxygenases and sequence analysis of the chlorocatechol dioxygenase region of Pseudomonas putida GJ31.

Journal of bacteriology ·Vol. 181 ·No. 4 ·1999-02-00 ·Pages 1309-18

Mars AE, Kingma J, Kaschabek SR, Reineke W, Janssen DB

Abstract

Pseudomonas putida GJ31 contains an unusual catechol 2,3-dioxygenase that converts 3-chlorocatechol and 3-methylcatechol, which enables the organism to use both chloroaromatics and methylaromatics for growth. A 3.1-kb region of genomic DNA of strain GJ31 containing the gene for this chlorocatechol 2,3-dioxygenase (cbzE) was cloned and sequenced. The cbzE gene appeared to be plasmid localized and was found in a region that also harbors genes encoding a transposase, a ferredoxin that was homologous to XylT, an open reading frame with similarity to a protein of a meta-cleavage pathway with unknown function, and a 2-hydroxymuconic semialdehyde dehydrogenase. CbzE was most similar to catechol 2,3-dioxygenases of the 2.C subfamily of type 1 extradiol dioxygenases (L. D. Eltis and J. T. Bolin, J. Bacteriol. 178:5930-5937, 1996). The substrate range and turnover capacity with 3-chlorocatechol were determined for CbzE and four related catechol 2,3-dioxygenases. The results showed that CbzE was the only enzyme that could productively convert 3-chlorocatechol. Besides, CbzE was less susceptible to inactivation by methylated catechols. Hybrid enzymes that were made of CzbE and the catechol 2, 3-dioxygenase of P. putida UCC2 (TdnC) showed that the resistance of CbzE to suicide inactivation and its substrate specificity were mainly determined by the C-terminal region of the protein.

MeSH Terms
Amino Acid Sequence Catechol 2,3-Dioxygenase Catechols/metabolism Cloning, Molecular Dioxygenases Genes, Bacterial Molecular Sequence Data Oxygenases/genetics,metabolism Plasmids Pseudomonas putida/enzymology,genetics Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Substrate Specificity
Chemicals
Catechols Recombinant Fusion Proteins 3-chlorocatechol Oxygenases Dioxygenases catechol oxygenase II Catechol 2,3-Dioxygenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mars A E
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands.
Kingma J
Kaschabek S R
Reineke W
Janssen D B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.4.1309-1318.1999
Published
1999-02-00
Pages
1309-18
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93510
Subset
IM
Databases
GENBANK
AF109307
External Links
PubMed source DOI: 10.1128/JB.181.4.1309-1318.1999 PMC Full Text View journal details
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