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PMID: 10781562 Published · ppublish English Journal Article

Altered stationary-phase response in a Borrelia burgdorferi rpoS mutant.

Journal of bacteriology ·Vol. 182 ·No. 10 ·2000-05-00 ·Pages 2909-18

Elias AF, Bono JL, Carroll JA, Stewart P, Tilly K, Rosa P

Abstract

The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrB(r) as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase-dependent resistance to 1 N NaCl, similar to the stationary-phase response reported for other bacteria. The B. burgdorferi rpoS mutant strain was less resistant to osmotic stress in stationary phase than the isogenic rpoS wild-type organism. The results indicate that the B. burgdorferi rpoS homolog influences protein composition and participates in stationary-phase-dependent osmotic resistance. This rpoS mutant will be useful for studying regulation of gene expression in response to changing environmental conditions.

MeSH Terms
Bacterial Proteins/genetics Borrelia burgdorferi Group/genetics,growth & development Genes, Bacterial Mutagenesis Osmosis Sigma Factor/genetics Sodium Chloride Transcription, Genetic
Chemicals
Bacterial Proteins Sigma Factor sigma factor KatF protein, Bacteria Sodium Chloride
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Elias A F
Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. [email protected]
Bono J L
Carroll J A
Stewart P
Tilly K
Rosa P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.10.2909-2918.2000
Email
[email protected]
Published
2000-05-00
Pages
2909-18
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC102002
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.182.10.2909-2918.2000 PMC Full Text View journal details
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