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PMID: 10872330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Codon-specific missense errors in vivo.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1351-6

Bouadloun F, Donner D, Kurland CG

Abstract

We have developed a simple method for measuring the missense substitution of amino acids at specified positions in proteins synthesized in vivo. We find that the frequency of cysteine substitution for the single arginine in Escherichia coli ribosomal protein L7/L12 is close to 10(-3) for wild-type bacteria, decreases to 4 x 10(-4) in streptomycin-resistant bacteria containing mutant S12 (rpsL), and is virtually unchanged in Ram bacteria containing mutant S4 (rpsD). We have also found that the frequency of the cysteine substitution for the single tryptophan in E. coli ribosomal protein S6 is 3-4 x 10(-3) for wild-type bacteria, decreases to 6 x 10(-4) in streptomycin-resistant bacteria and is elevated to nearly 10(-2) in Ram bacteria.

MeSH Terms
Amino Acid Substitution Arginine/genetics Codon Cysteine/genetics Escherichia coli/genetics Escherichia coli Proteins Mutation, Missense Ribosomal Protein S9 Ribosomal Proteins/genetics Tryptophan/genetics
Chemicals
Codon Escherichia coli Proteins Ribosomal Protein S9 Ribosomal Proteins RpsI protein, E coli ribosomal protein L7-L12 Tryptophan Arginine Cysteine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bouadloun F
Department of Molecular Biology, University of Uppsala, Sweden.
Donner D
Kurland C G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1351-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555282
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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