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PMID: 10970883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Degradation of Drosophila PIM regulates sister chromatid separation during mitosis.

Genes & development ·Vol. 14 ·No. 17 ·2000-09-01 ·Pages 2192-205

Leismann O, Herzig A, Heidmann S, Lehner CF

Abstract

Drosophila Pimples (PIM) and Three rows (THR) are required for sister chromatid separation in mitosis. PIM accumulates during interphase and is degraded rapidly during mitosis. This degradation is dependent on a destruction box similar to that of B-type cyclins. Nondegradable PIM with a mutant destruction box can rescue sister chromatid separation in pim mutants but only when expressed at low levels. Higher levels of nondegradable PIM, as well as overexpression of wild-type PIM, inhibit sister chromatid separation. Moreover, cells arrested in mitosis before sister chromatid separation (by colcemid or by mutations in fizzy/CDC20) fail to degrade PIM. Thus, although not related by primary sequence, PIM has intriguing functional similarities to the securin proteins of budding yeast, fission yeast, and vertebrates. Whereas these securins are known to form a complex with separins, we show that PIM associates in vivo with THR, which does not contain the conserved separin domain.

MeSH Terms
Amino Acid Sequence Animals Animals, Genetically Modified Antineoplastic Agents, Phytogenic/pharmacology Cdc20 Proteins Cell Cycle Proteins/chemistry,genetics,metabolism Chromatids/genetics,ultrastructure Cyclin B/genetics,metabolism DNA-Binding Proteins Demecolcine/pharmacology Drosophila/genetics Drosophila Proteins Epitopes Fungal Proteins/metabolism Immunoblotting Insect Proteins/chemistry,genetics,metabolism Mitosis/drug effects,genetics Molecular Sequence Data Mutagenesis, Site-Directed Precipitin Tests Protein Binding Protein Structure, Tertiary Proto-Oncogene Proteins c-myc/metabolism Saccharomyces cerevisiae Proteins Transcription Factors/metabolism
Chemicals
Antineoplastic Agents, Phytogenic Cdc20 Proteins Cell Cycle Proteins CycB protein, Drosophila Cyclin B DNA-Binding Proteins Drosophila Proteins Epitopes Fungal Proteins GAL4 protein, S cerevisiae Insect Proteins PIM protein, Drosophila Proto-Oncogene Proteins c-myc Saccharomyces cerevisiae Proteins Transcription Factors fzy protein, Drosophila thr protein, Drosophila Demecolcine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leismann O
Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany.
Herzig A
Heidmann S
Lehner C F
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.176700
Published
2000-09-01
Pages
2192-205
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316890
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.176700 PMC Full Text View journal details
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