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PMID: 11062253 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles.

The Journal of cell biology ·Vol. 151 ·No. 3 ·2000-10-30 ·Pages 507-18

Brügger B, Sandhoff R, Wegehingel S, Gorgas K, Malsam J, Helms JB, Lehmann WD, Nickel W, Wieland FT

Abstract

In higher eukaryotes, phospholipid and cholesterol synthesis occurs mainly in the endoplasmic reticulum, whereas sphingomyelin and higher glycosphingolipids are synthesized in the Golgi apparatus. Lipids like cholesterol and sphingomyelin are gradually enriched along the secretory pathway, with their highest concentration at the plasma membrane. How a cell succeeds in maintaining organelle-specific lipid compositions, despite a steady flow of incoming and outgoing transport carriers along the secretory pathway, is not yet clear. Transport and sorting along the secretory pathway of both proteins and most lipids are thought to be mediated by vesicular transport, with coat protein I (COPI) vesicles operating in the early secretory pathway. Although the protein constituents of these transport intermediates are characterized in great detail, much less is known about their lipid content. Using nano-electrospray ionization tandem mass spectrometry for quantitative lipid analysis of COPI-coated vesicles and their parental Golgi membranes, we find only low amounts of sphingomyelin and cholesterol in COPI-coated vesicles compared with their donor Golgi membranes, providing evidence for a significant segregation from COPI vesicles of these lipids. In addition, our data indicate a sorting of individual sphingomyelin molecular species. The possible molecular mechanisms underlying this segregation, as well as implications on COPI function, are discussed.

MeSH Terms
Animals Biological Transport Brain/cytology,metabolism CHO Cells COP-Coated Vesicles/chemistry,metabolism Cattle Cholesterol/analysis,metabolism Cricetinae Golgi Apparatus/chemistry,metabolism Guanosine Triphosphate/metabolism Hydrolysis Intracellular Membranes/chemistry,metabolism Liver/cytology,metabolism Phosphatidylcholines/analysis Rats Spectrometry, Mass, Electrospray Ionization Sphingomyelins/analysis,metabolism
Chemicals
Phosphatidylcholines Sphingomyelins Guanosine Triphosphate Cholesterol
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Brügger B
Biochemie-Zentrum Heidelberg (BZH), Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany.
Sandhoff R
Wegehingel S
Gorgas K
Malsam J
Helms J B
Lehmann W D
Nickel W
Wieland F T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.151.3.507
Published
2000-10-30
Pages
507-18
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2185577
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.151.3.507 PMC Full Text View journal details
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