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PMID: 1107305 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inhibition of nucleoside Q formation in transfer ribonucleic acid during methionine starvation of relaxed-control Escherichia coli.

Journal of bacteriology ·Vol. 125 ·No. 1 ·1976-01-00 ·Pages 205-10

Katze JR, Mosteller RD

Abstract

The elution profiles of Asp-tRNA from unstarved and starved cultures of a relaxed-control (Rel-) strain of Escherichia coli were compared by reversed-phase chromatography. Methionine starvation results in the appearance of several additional species of Asp-tRNA which are not observed with starvation for leucine or histidine. By the criterion of cyanogen bromide-effected shifts in chromatographic elution position, a large portion of the tRNAAsp synthesized in methionine-starved cells lacks the normal Q nucleoside. By the same criterion, virtually all of the tRNAAsp from unstarved, leucine-starved, and histidine-starved cells contain Q. We conclude that methionine starvation prevents the formation of the norma Q nucleoside in Rel- E. coli.

MeSH Terms
Aspartic Acid/metabolism Cyanogen Bromide/pharmacology Escherichia coli/metabolism Guanosine/analogs & derivatives,biosynthesis Histidine/metabolism Leucine/metabolism Methionine/metabolism RNA, Bacterial/biosynthesis RNA, Transfer/biosynthesis
Chemicals
RNA, Bacterial Guanosine Aspartic Acid Histidine RNA, Transfer Methionine Leucine Cyanogen Bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Katze J R
Mosteller R D
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.125.1.205-210.1976
Published
1976-01-00
Pages
205-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233353
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.125.1.205-210.1976 PMC Full Text View journal details
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