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PMID: 11073991 Published · ppublish English Journal Article

[URE3] prion propagation in Saccharomyces cerevisiae: requirement for chaperone Hsp104 and curing by overexpressed chaperone Ydj1p.

Molecular and cellular biology ·Vol. 20 ·No. 23 ·2000-12-00 ·Pages 8916-22

Moriyama H, Edskes HK, Wickner RB

Abstract

The [URE3] nonchromosomal genetic element is an infectious form (prion) of the Ure2 protein, apparently a self-propagating amyloidosis. We find that an insertion mutation or deletion of HSP104 results in inability to propagate the [URE3] prion. Our results indicate that Hsp104 is a common factor in the maintenance of two independent yeast prions. However, overproduction of Hsp104 does not affect the stability of [URE3], in contrast to what is found for the [PSI(+)] prion, which is known to be cured by either overproduction or deficiency of Hsp104. Like Hsp104, the Hsp40 class chaperone Ydj1p, with the Hsp70 class Ssa1p, can renature proteins. We find that overproduction of Ydj1p results in a gradual complete loss of [URE3]. The involvement of protein chaperones in the propagation of [URE3] indicates a role for protein conformation in inheritance.

MeSH Terms
Gene Deletion Genes, Fungal Genetic Complementation Test HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins/genetics,metabolism Heat-Shock Proteins/genetics,metabolism Models, Biological Molecular Chaperones/metabolism Mutagenesis, Insertional Prions/metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Chemicals
HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Molecular Chaperones Prions Saccharomyces cerevisiae Proteins YDJ1 protein, S cerevisiae HsP104 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moriyama H
Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830, USA.
Edskes H K
Wickner R B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.23.8916-8922.2000
Published
2000-12-00
Pages
8916-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86546
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.23.8916-8922.2000 PMC Full Text View journal details
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