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PMID: 110783 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.

Journal of bacteriology ·Vol. 139 ·No. 1 ·1979-07-00 ·Pages 270-9

Ganesan AT

Abstract

In Bacillus subtilis the ability to take up deoxyribonucleic acid (DNA) and undergo genetic transformation may coincide with the induction of defective phage(s) and the expression of possibly related cryptic genes. A restriction-modification enzyme system appears to be expressed. Targets of the restriction activity on the DNA can be blocked my methylation catalyzed by the methyl transferase. It is shown that cellular DNA becomes progressively methylated and reaches the maxium level during the peak of competency. Deoxycytidine residues of both incoming donor and resident DNA are methylated. The possible participation of these enzymes in recombination and the general role of cryptic genes in inducible functions are discussed.

MeSH Terms
Bacillus subtilis/enzymology,genetics DNA (Cytosine-5-)-Methyltransferases/biosynthesis DNA, Bacterial/metabolism Deoxycytidine/metabolism Enzyme Induction Methylation Methyltransferases/biosynthesis Recombination, Genetic Transformation, Bacterial
Chemicals
DNA, Bacterial Deoxycytidine Methyltransferases DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ganesan A T
References (36)
36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.1.270-279.1979
Published
1979-07-00
Pages
270-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216855
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.1.270-279.1979 PMC Full Text View journal details
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