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PMID: 11078522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The human SWI/SNF-B chromatin-remodeling complex is related to yeast rsc and localizes at kinetochores of mitotic chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 24 ·2000-11-21 ·Pages 13015-20

Xue Y, Canman JC, Lee CS, Nie Z, Yang D, Moreno GT, Young MK, Salmon ED, Wang W

Abstract

The SWI/SNF family of chromatin-remodeling complexes facilitates gene expression by helping transcription factors gain access to their targets in chromatin. SWI/SNF and Rsc are distinctive members of this family from yeast. They have similar protein components and catalytic activities but differ in biological function. Rsc is required for cell cycle progression through mitosis, whereas SWI/SNF is not. Human complexes of this family have also been identified, which have often been considered related to yeast SWI/SNF. However, all human subunits identified to date are equally similar to components of both SWI/SNF and Rsc, leaving open the possibility that some or all of the human complexes are rather related to Rsc. Here, we present evidence that the previously identified human SWI/SNF-B complex is indeed of the Rsc type. It contains six components conserved in both Rsc and SWI/SNF. Importantly, it has a unique subunit, BAF180, that harbors a distinctive set of structural motifs characteristic of three components of Rsc. Of the two mammalian ATPases known to be related to those in the yeast complexes, human SWI/SNF-B contains only the homolog that functions like Rsc during cell growth. Immunofluorescence studies with a BAF180 antibody revealed that SWI/SNF-B localizes at the kinetochores of chromosomes during mitosis. Our data suggest that SWI/SNF-B and Rsc represent a novel subfamily of chromatin-remodeling complexes conserved from yeast to human, and could participate in cell division at kinetochores of mitotic chromosomes.

MeSH Terms
Adenosine Triphosphatases/chemistry,genetics,metabolism Amino Acid Sequence Animals Chickens Chromosomes, Human/genetics,ultrastructure DNA Helicases DNA-Binding Proteins/chemistry,genetics,metabolism Humans Kinetochores/physiology Leucine Zippers Microtubules/physiology Mitosis Molecular Sequence Data Nuclear Proteins Protein Subunits Recombinant Fusion Proteins/chemistry,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Transcription Factors/chemistry,genetics,metabolism
Chemicals
DNA-Binding Proteins Nuclear Proteins Protein Subunits RSC complex, S cerevisiae Recombinant Fusion Proteins SMARCA1 protein, human SMARCA2 protein, human Saccharomyces cerevisiae Proteins Transcription Factors Adenosine Triphosphatases SMARCA4 protein, human SNF2 protein, S cerevisiae DNA Helicases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Xue Y
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, TRIAD Center Room 4000, Baltimore, MD 21224, USA.
Canman J C
Lee C S
Nie Z
Yang D
Moreno G T
Young M K
Salmon E D
Wang W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.240208597
Published
2000-11-21
Pages
13015-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC27170
Subset
IM
Grants
FDA HHS · GENBANK/A197569 · United States
External Links
PubMed source DOI: 10.1073/pnas.240208597 PMC Full Text View journal details
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