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PMID: 9154831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression.

Molecular and cellular biology ·Vol. 17 ·No. 6 ·1997-06-00 ·Pages 3323-34

Cao Y, Cairns BR, Kornberg RD, Laurent BC

Abstract

Several eukaryotic multiprotein complexes, including the Saccharomyces cerevisiae Snf/Swi complex, remodel chromatin for transcription. In contrast to the Snf/Swi proteins, Sfh1p, a new Snf5p paralog, is essential for viability. The evolutionarily conserved domain of Sfh1p is sufficient for normal function, and Sfh1p interacts functionally and physically with an essential Snf2p paralog in a novel nucleosome-restructuring complex called RSC (for remodels the structure of chromatin). A temperature-sensitive sfh1 allele arrests cells in the G2/M phase of the cell cycle, and the Sfh1 protein is specifically phosphorylated in the G1 phase. Together, these results demonstrate a link between chromatin remodeling and progression through the cell division cycle, providing genetic clues to possible targets for RSC function.

MeSH Terms
Adenosine Triphosphatases Adenosine Triphosphate/genetics,metabolism Amino Acid Sequence Animals Caenorhabditis elegans Cell Cycle/physiology Cell Cycle Proteins/genetics,physiology Chromatin/metabolism Chromosomal Proteins, Non-Histone Conserved Sequence DNA Helicases DNA-Binding Proteins/genetics,metabolism,physiology Drosophila melanogaster Fungal Proteins/metabolism Humans Molecular Sequence Data Nuclear Proteins Nucleosomes/metabolism Phenotype Phosphorylation SMARCB1 Protein Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism,physiology
Chemicals
Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins Fungal Proteins Nuclear Proteins Nucleosomes SFH1 protein, S. cerevisiae SMARCA2 protein, human SMARCB1 Protein SMARCB1 protein, human SNF5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Adenosine Triphosphate Adenosine Triphosphatases SMARCA4 protein, human SNF2 protein, S cerevisiae STH1 protein, S cerevisiae DNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cao Y
Department of Microbiology and Immunology and Morse Institute of Molecular Biology and Genetics, State University of New York, Brooklyn 11203, USA.
Cairns B R
Kornberg R D
Laurent B C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.6.3323
Published
1997-06-00
Pages
3323-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232185
Subset
IM
Grants
NIGMS NIH HHS · GM36659 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.6.3323 PMC Full Text View journal details
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