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PMID: 11121053 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

HO endonuclease-induced recombination in yeast meiosis resembles Spo11-induced events.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 26 ·2000-12-19 ·Pages 14500-5

Malkova A, Klein F, Leung WY, Haber JE

Abstract

In meiosis, gene conversions are accompanied by higher levels of crossing over than in mitotic cells. To determine whether the special properties of meiotic recombination can be attributed to the way in which Spo11p creates double-strand breaks (DSBs) at special hot spots in Saccharomyces cerevisiae, we expressed the site-specific HO endonuclease in meiotic cells. We could therefore compare HO-induced recombination in a well-defined region both in mitosis and meiosis, as well as compare Spo11p- and HO-induced meiotic events. HO-induced gene conversions in meiosis were accompanied by crossovers at the same high level (52%) as Spo11p-induced events. Moreover, HO-induced crossovers were reduced 3-fold by a msh4Delta mutation that similarly affects Spo11p-promoted events. In a spo11Delta diploid, where the only DSB is made by HO, crossing over was significantly higher (27%) than in mitotic cells (</=7%). This single meiotic DSB failed to induce the formation of a synaptonemal complex. We also show that HO-induced gene conversion tract lengths are shorter in meiotic than in mitotic cells. We conclude that a hallmark of meiotic recombination, the production of crossovers, is independent of the nature of Spo11p-generated DSBs at special hotspots, but some functions of Spo11p are required in trans to achieve maximum crossing over.

MeSH Terms
Chromatids Crossing Over, Genetic DNA Topoisomerases, Type II/genetics,metabolism DNA-Binding Proteins Deoxyribonucleases, Type II Site-Specific/genetics,metabolism Endodeoxyribonucleases Esterases/genetics,metabolism Fungal Proteins/genetics,metabolism Gene Conversion Meiosis/physiology Mitosis/physiology Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
DNA-Binding Proteins Fungal Proteins MSH4 protein, S cerevisiae SPO13 protein, S cerevisiae Saccharomyces cerevisiae Proteins Endodeoxyribonucleases Esterases meiotic recombination protein SPO11 HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Malkova A
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA.
Klein F
Leung W Y
Haber J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.97.26.14500
Published
2000-12-19
Pages
14500-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18948
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.97.26.14500 PMC Full Text View journal details
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