Home LiteratureArticle Details
PMID: 11607193 Published · ppublish English Journal Article

Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 12 ·1991-06-15 ·Pages 5355-9

Wolk CP, Cai Y, Panoff JM

Abstract

Anabaena, a filamentous cyanobacterium, is of developmental interest because, when deprived of fixed nitrogen, it shows patterned differentiation of N2-fixing cells called heterocysts. To help elucidate its early responses to a decrease in nitrogen, we used a derivative of transposon Tn5 to generate transcriptional fusions of promoterless bacterial luciferase genes, luxAB, to the Anabaena genome. Genes that responded to removal of fixed nitrogen or to other environmental shifts by increased or decreased transcription were identified by monitoring the luminescence of colonies from transposon-generated libraries. The Tn5 derivative transposed in Anabaena at ca. 1-4 x 10(-5) per cell and permitted high-resolution mapping of its position and orientation in the genome and facile cloning of contiguous genomic DNA.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wolk C P
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA.
Cai Y
Panoff J M
References (46)
46 references, click to expand
  1. New Anabaena and Nostoc cyanophages from sewage settling ponds.
    Virology. 1981 Oct 15;114(1):236-46 PMID: 6269286
  2. Organization and transcription of genes important in Anabaena heterocyst differentiation.
    Ann Microbiol (Paris). 1983 Jul-Aug;134B(1):181-93 PMID: 6139054
  3. Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri.
    Cell. 1983 Mar;32(3):773-81 PMID: 6831560
  4. Control of gene expression in blue-green algae.
    Nature. 1975 Feb 20;253(5493):650-1 PMID: 803643
  5. Mutants of Anabaena cylindrica altered in heterocyst spacing.
    Arch Microbiol. 1975 May 5;103(3):219-23 PMID: 807173
  6. Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity.
    J Bacteriol. 1978 Nov;136(2):688-92 PMID: 101529
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  9. Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120.
    J Bacteriol. 1991 Mar;173(6):1879-85 PMID: 1900504
  10. Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts.
    J Bacteriol. 1990 Jun;172(6):3131-7 PMID: 2111805
  11. Construction of lacZ promoter probe vectors for use in Synechococcus: application to the identification of CO2-regulated promoters.
    Gene. 1990 May 31;90(1):43-9 PMID: 2116369
  12. Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena.
    EMBO J. 1990 Oct;9(10):3379-88 PMID: 2120040
  13. Signal transduction in bacteria.
    Nature. 1990 Mar 29;344(6265):395-400 PMID: 2157156
  14. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences.
    J Bacteriol. 1990 Jun;172(6):3138-45 PMID: 2160938
  15. A novel immune system against bacteriophage infection using complementary RNA (micRNA).
    Nature. 1985 Jun 13-19;315(6020):601-3 PMID: 2409446
  16. Major cold shock protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1990 Jan;87(1):283-7 PMID: 2404279
  17. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
    J Bacteriol. 1990 Nov;172(11):6568-72 PMID: 2172217
  18. Promoter determining the timing and spatial localization of transcription of a cloned Streptomyces coelicolor gene encoding a spore-associated polypeptide.
    J Bacteriol. 1988 Apr;170(4):1895-901 PMID: 2450872
  19. Effect of dam methylation on Tn5 transposition.
    J Mol Biol. 1988 Jan 5;199(1):35-45 PMID: 2451025
  20. Low-temperature effects on cyanobacterial membranes.
    J Bioenerg Biomembr. 1989 Feb;21(1):61-75 PMID: 2496118
  21. Novel water stress protein from a desiccation-tolerant cyanobacterium. Purification and partial characterization.
    J Biol Chem. 1989 Jul 25;264(21):12546-53 PMID: 2501307
  22. Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.
    J Bacteriol. 1989 Nov;171(11):5940-8 PMID: 2509424
  23. Factors affecting transposition activity of IS50 and Tn5 ends.
    Gene. 1989;76(2):207-13 PMID: 2546858
  24. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in gram-negative bacteria.
    Gene. 1989 Aug 1;80(1):161-9 PMID: 2551782
  25. Tn5 mutagenesis of Anabaena sp. strain PCC 7120: isolation of a new mutant unable to grow without combined nitrogen.
    J Bacteriol. 1989 Oct;171(10):5759-61 PMID: 2551892
  26. Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen.
    J Bacteriol. 1988 Mar;170(3):1239-44 PMID: 2830231
  27. Restriction analysis and quantitative estimation of methylated bases of filamentous and unicellular cyanobacterial DNAs.
    J Bacteriol. 1988 Apr;170(4):1934-9 PMID: 2832390
  28. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.
    Gene. 1988 Aug 15;68(1):119-38 PMID: 2851487
  29. Construction of a Tn5 derivative encoding bioluminescence and its introduction in Pseudomonas, Agrobacterium and Rhizobium.
    Mol Gen Genet. 1988 Jul;213(1):50-5 PMID: 2851709
  30. Nucleotide sequence and gene organization of ColE1 DNA.
    J Biol Chem. 1985 Jul 25;260(15):8925-35 PMID: 2991225
  31. Expression of the Escherichia coli lacZ gene on a plasmid vector in a cyanobacterium.
    Science. 1985 Nov 15;230(4727):805-7 PMID: 2997920
  32. IS10 transposition is regulated by DNA adenine methylation.
    Cell. 1985 Nov;43(1):117-30 PMID: 3000598
  33. Uses of lac fusions for the study of biological problems.
    Microbiol Rev. 1985 Dec;49(4):398-418 PMID: 3005818
  34. A global analysis of developmentally regulated genes in Myxococcus xanthus.
    Dev Biol. 1986 Sep;117(1):252-66 PMID: 3017794
  35. Expression of Tn5-encoded streptomycin resistance in E. coli.
    Mol Gen Genet. 1986 Sep;204(3):404-9 PMID: 3020378
  36. Plasmid vector pBR322 and its special-purpose derivatives--a review.
    Gene. 1986;50(1-3):3-40 PMID: 3034735
  37. High efficiency transformation of E. coli by high voltage electroporation.
    Nucleic Acids Res. 1988 Jul 11;16(13):6127-45 PMID: 3041370
  38. The nucleotide sequence of pACYC184.
    Nucleic Acids Res. 1988 Jan 11;16(1):355 PMID: 3340533
  39. Nucleotide sequence of the LuxA and LuxB genes of the bioluminescent marine bacterium Vibrio fischeri.
    Nucleic Acids Res. 1988 Jan 25;16(2):777 PMID: 3340562
  40. Visualizing gene expression in time and space in the filamentous bacterium Streptomyces coelicolor.
    Science. 1988 May 6;240(4853):768-72 PMID: 3363358
  41. A single-photon imaging system for the simultaneous quantitation of luminescent emissions from multiple samples.
    Anal Biochem. 1988 Feb 1;168(2):462-9 PMID: 3364741
  42. Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts.
    Science. 1985 Nov 1;230(4725):550-3 PMID: 3931221
  43. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  44. Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5816-20 PMID: 6091110
  45. Bacterial regulation: global regulatory networks.
    Annu Rev Genet. 1984;18:415-41 PMID: 6099091
  46. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1561-5 PMID: 6324204
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.12.5355
Published
1991-06-15
Pages
5355-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51871
External Links
PubMed source DOI: 10.1073/pnas.88.12.5355 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2