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PMID: 11701886 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repression of the defense gene PR-10a by the single-stranded DNA binding protein SEBF.

The Plant cell ·Vol. 13 ·No. 11 ·2001-11-00 ·Pages 2525-37

Boyle B, Brisson N

Abstract

The potato pathogenesis-related gene PR-10a is transcriptionally activated in response to pathogen infection or elicitor treatment. Characterization of the cis-acting elements of the PR-10a promoter revealed the presence of a silencing element between residues -52 and -27 that contributes to transcriptional regulation. In this study, we have isolated a silencing element binding factor (SEBF) from potato tuber nuclei that binds to the coding strand of the silencing element in a sequence-specific manner. The consensus binding site of SEBF, PyTGTCNC, is present in a number of PR genes and shows striking similarity to the auxin response element. Mutational analysis of the PR-10a promoter revealed an inverse correlation between the in vitro binding of SEBF and the expression of PR-10a. SEBF was purified to homogeneity from potato tubers, and sequencing of the N terminus of the protein led to the isolation of a cDNA clone. Sequence analysis revealed that SEBF is homologous with chloroplast RNA binding proteins that possess consensus sequence-type RNA binding domains characteristic of heterogeneous nuclear ribonucleoproteins (hnRNPs). Overexpression of SEBF in protoplasts repressed the activity of a PR-10a reporter construct in a silencing element-dependent manner, confirming the role of SEBF as a transcriptional repressor.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/metabolism,physiology Chloroplasts/metabolism Cloning, Molecular DNA Primers DNA, Single-Stranded/genetics DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Plant Molecular Sequence Data Nuclear Proteins/genetics Plant Leaves/physiology Plant Proteins Polymerase Chain Reaction Promoter Regions, Genetic RNA-Binding Proteins/genetics,metabolism Recombinant Proteins/metabolism Repressor Proteins/genetics,metabolism Sequence Alignment Solanum tuberosum/genetics Transcription, Genetic
Chemicals
DNA Primers DNA, Single-Stranded DNA-Binding Proteins Nuclear Proteins PBF-2 protein, Solanum tuberosum Plant Proteins RNA-Binding Proteins Recombinant Proteins Repressor Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boyle B
Department of Biochemistry, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
Brisson N
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
Published
2001-11-00
Pages
2525-37
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139469
Subset
IM
Databases
GENBANK
AF389431
External Links
PubMed source PMC Full Text View journal details
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